View source for 5wz2


==Crystal structure of Zika virus NS5 methyltransferase bound to SAM and RNA analogue (m7GpppA)==
<StructureSection load='5wz2' size='340' side='right'caption='[[5wz2]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wz2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Zika_virus Zika virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WZ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WZ2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTA:P1-7-METHYLGUANOSINE-P3-ADENOSINE-5,5-TRIPHOSPHATE'>GTA</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wz2 OCA], [https://pdbe.org/5wz2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wz2 RCSB], [https://www.ebi.ac.uk/pdbsum/5wz2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wz2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A109PRQ3_ZIKV A0A109PRQ3_ZIKV] Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.[ARBA:ARBA00003504]  Serine protease subunit NS2B: Required cofactor for the serine protease function of NS3.[PROSITE-ProRule:PRU00859]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Zika virus (ZIKV) has emerged as major health concern, as ZIKV infection has been shown to be associated with microcephaly, severe neurological disease and possibly male sterility. As the largest protein component within the ZIKV replication complex, NS5 plays key roles in the life cycle and survival of the virus through its N-terminal methyltransferase (MTase) and C-terminal RNA-dependent RNA polymerase (RdRp) domains. Here, we present the crystal structures of ZIKV NS5 MTase in complex with an RNA cap analogue (m7GpppA) and the free NS5 RdRp. We have identified the conserved features of ZIKV NS5 MTase and RdRp structures that could lead to development of current antiviral inhibitors being used against flaviviruses, including dengue virus and West Nile virus, to treat ZIKV infection. These results should inform and accelerate the structure-based design of antiviral compounds against ZIKV.

The crystal structure of Zika virus NS5 reveals conserved drug targets.,Duan W, Song H, Wang H, Chai Y, Su C, Qi J, Shi Y, Gao GF EMBO J. 2017 Apr 3;36(7):919-933. doi: 10.15252/embj.201696241. Epub 2017 Mar 2. PMID:28254839<ref>PMID:28254839</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5wz2" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Zika virus]]
[[Category: Duan W]]
[[Category: Gao GF]]
[[Category: Qi J]]
[[Category: Shi Y]]
[[Category: Song H]]