View source for 5x5l


==Crystal structure of response regulator AdeR DNA binding domain in complex with an intercistronic region==
<StructureSection load='5x5l' size='340' side='right'caption='[[5x5l]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5x5l]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X5L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5X5L FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5x5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x5l OCA], [https://pdbe.org/5x5l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5x5l RCSB], [https://www.ebi.ac.uk/pdbsum/5x5l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5x5l ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/E1A0Z5_ACIBA E1A0Z5_ACIBA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
AdeR-AdeS is a two-component regulatory system, which controls expression of the adeABC efflux pump involved in Acinetobacter baumannii multidrug resistance. AdeR is a response regulator consisting of an N-terminal receiver domain and a C-terminal DNA-binding-domain. AdeR binds to a direct-repeat DNA in the intercistronic region between adeR and adeABC. We demonstrate a markedly high affinity binding between unphosphorylated AdeR and DNA with a dissociation constant of 20 nM. In addition, we provide a 2.75 A crystal structure of AdeR DNA-binding-domain complexed with the intercistronic DNA. This structure shows that the alpha3 and beta hairpin formed by beta5-beta6 interacts with the major and minor groove of the DNA, which in turn leads to the introduction of a bend. The AdeR receiver domain structure revealed a dimerization motif mediated by a gearwheel-like structure involving the D108F109-R122 motif through cation pi stack interaction. The structure of AdeR receiver domain bound with magnesium indicated a conserved Glu19Asp20-Asp63 magnesium-binding motif, and revealed that the potential phosphorylation site Asp63OD1 forms a hydrogen bond with Lys112. We thus dissected the mechanism of how AdeR recognizes the intercistronic DNA, which leads to a diverse mode of response regulation. Unlocking the AdeRS mechanism provides ways to circumvent A. baumannii antibiotic resistance.

Mechanistic insight into how multidrug resistant Acinetobacter baumannii response regulator AdeR recognizes an intercistronic region.,Wen Y, Ouyang Z, Yu Y, Zhou X, Pei Y, Devreese B, Higgins PG, Zheng F Nucleic Acids Res. 2017 Sep 19;45(16):9773-9787. doi: 10.1093/nar/gkx624. PMID:28934482<ref>PMID:28934482</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5x5l" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Acinetobacter baumannii]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Wen Y]]