View source for 5xbd


==Disulfide-constrained Wound Healing Peptide Derived from Pereskia bleo==
<StructureSection load='5xbd' size='340' side='right'caption='[[5xbd]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xbd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leuenbergeria_bleo Leuenbergeria bleo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XBD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XBD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xbd OCA], [https://pdbe.org/5xbd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xbd RCSB], [https://www.ebi.ac.uk/pdbsum/5xbd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xbd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2R2JFU8_9CARY A0A2R2JFU8_9CARY] 
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== Publication Abstract from PubMed ==
Cysteine-rich peptides (CRPs) play important host-defense roles in plants. However, information concerning CRPs in the Cactaceae (cactus) family is limited, with only a single cactus-derived CRP described to date. Here, we report the identification of 15 novel CRPs with three different precursor architectures, bleogens pB1-15 from Pereskia bleo of the Cactaceae family. By combining proteomic and transcriptomic methods, we showed that the prototype, bleogen pB1, contained 36 amino acid residues, a six-cysteine motif typical of the six-cysteine-hevein-like peptide (6C-HLP) family, and a type I two-domain precursor consisting of an endoplasmic reticulum (ER) and a mature domain. In contrast, the precursors of the other 14 bleogens contained a type II three-domain architecture with a propeptide domain inserted between the ER and the mature bleogen domain. Four of these 14 bleogens display a third type of architecture with a tandemly repeating bleogen domain. A search of the Onekp database revealed that &lt;1% plant species possess three different precursor architectures for the biosynthesis of 6C-HLPs, including Lophophora williamsii, Pereskia aculeate, Portulaca cryptopetala, Portulaca oleracea, Portulaca suffruticosa, and Talinum sp. NMR analysis confirmed that bleogen pB1 has cystine-knot disulfide connectivity as well as a two-beta-sheet and a four-loop structural fold that is similar to other 6C-HLPs. Sequence analysis, structural studies, and in silico modeling revealed that bleogen pB1 has a cation-polar-cation motif, a signature heparin-binding motif that was confirmed by heparin affinity chromatography. Cell-based assays showed that bleogen pB1 is non-toxic to mammalian cells but functions as an anti-Candida peptide. Taken together, our findings provide insight into the occurrence, functions and precursor architectures of CRPs in the cactus family.

Bleogens: Cactus-Derived Anti-Candida Cysteine-Rich Peptides with Three Different Precursor Arrangements.,Loo S, Kam A, Xiao T, Tam JP Front Plant Sci. 2017 Dec 22;8:2162. doi: 10.3389/fpls.2017.02162. eCollection, 2017. PMID:29312404<ref>PMID:29312404</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Leuenbergeria bleo]]
[[Category: Tam JP]]
[[Category: Xiao T]]