View source for 5xbs


==Peroxiredoxin from Aeropyrum pernix (6m mutant)==
<StructureSection load='5xbs' size='340' side='right'caption='[[5xbs]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xbs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeropyrum_pernix_K1 Aeropyrum pernix K1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XBS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XBS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.51&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xbs OCA], [https://pdbe.org/5xbs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xbs RCSB], [https://www.ebi.ac.uk/pdbsum/5xbs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xbs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TDXH_AERPE TDXH_AERPE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Peroxiredoxin from Pyrococcus horikoshii (PhPrx) is a decameric protein formed by ring-type assembly of five dimers. To engineer the quaternary structure of PhPrx, we created a mutant PhPrx (PhPrx6m) by introducing six point mutations designed to dissociate PhPrx into dimers. Although PhPrx6m was a dimer in solution, the six dimers assembled into a dodecamer following crystallization. In the crystal structure, PhPrx6m was overoxidized, and the peroxidatic cysteine was in sulfonic acid form and two cysteines in the C-terminal region were linked by an intramolecular disulfide bond. Thus, we characterized the wild-type PhPrx overoxidized by hydrogen peroxide (PhPrxPer). Analytical ultracentrifugation showed that PhPrxPer had a higher molecular mass in solution than PhPrx. This was confirmed by analysis of the crystal structure of PhPrxPer, which was found to form a ring-type dodecamer composed of six dimers. The monomeric structures of PhPrx6m and PhPrxPer differed from that of PhPrx in the relative orientation of two domains, reflecting the number of dimers in the ring-type assembly. Unlike PhPrx, homologous peroxiredoxin from Aeropyrum pernix (ApPrx) did not undergo hexameric association. This property can be explained by the stronger connection between the two domains in ApPrx due to its C-terminal extension relative to PhPrx.

Alteration of molecular assembly of peroxiredoxins from hyperthermophilic archaea.,Nakamura T, Oshima M, Yasuda M, Shimamura A, Morita J, Uegaki K J Biochem. 2017 Dec 1;162(6):415-422. doi: 10.1093/jb/mvx045. PMID:28992240<ref>PMID:28992240</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xbs" style="background-color:#fffaf0;"></div>

==See Also==
*[[Peroxiredoxin 3D structures|Peroxiredoxin 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Aeropyrum pernix K1]]
[[Category: Large Structures]]
[[Category: Nakamura T]]
[[Category: Uegaki K]]