View source for 5xbw


==The structure of BrlR==
<StructureSection load='5xbw' size='340' side='right'caption='[[5xbw]], [[Resolution|resolution]] 3.11&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xbw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XBW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.109&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xbw OCA], [https://pdbe.org/5xbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xbw RCSB], [https://www.ebi.ac.uk/pdbsum/5xbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xbw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9HUT5_PSEAE Q9HUT5_PSEAE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The virulence factor pyocyanin and the intracellular second messenger cyclic diguanylate monophosphate (c-di-GMP) play key roles in regulating biofilm formation and multi-drug efflux pump expression in Pseudomonas aeruginosa. However, the crosstalk between these two signaling pathways remains unclear. Here we show that BrlR (PA4878), previously identified as a c-di-GMP responsive transcriptional regulator, acts also as a receptor for pyocyanin. Crystal structures of free BrlR and c-di-GMP-bound BrlR reveal that the DNA-binding domain of BrlR contains two separate c-di-GMP binding sites, both of which are involved in promoting brlR expression. In addition, we identify a pyocyanin-binding site on the C-terminal multidrug-binding domain based on the structure of the BrlR-C domain in complex with a pyocyanin analog. Biochemical analysis indicates that pyocyanin enhances BrlR-DNA binding and brlR expression in a concentration-dependent manner.

BrlR from Pseudomonas aeruginosa is a receptor for both cyclic di-GMP and pyocyanin.,Wang F, He Q, Yin J, Xu S, Hu W, Gu L Nat Commun. 2018 Jul 2;9(1):2563. doi: 10.1038/s41467-018-05004-y. PMID:29967320<ref>PMID:29967320</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xbw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Gu L]]
[[Category: Qing H]]
[[Category: Wang F]]