View source for 5xc4


==Crystal structure of GH45 endoglucanase EG27II at pH4.0, in complex with cellobiose==
<StructureSection load='5xc4' size='340' side='right'caption='[[5xc4]], [[Resolution|resolution]] 1.42&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xc4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ampullaria_crossean Ampullaria crossean]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XC4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.42&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PRD_900005:beta-cellobiose'>PRD_900005</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xc4 OCA], [https://pdbe.org/5xc4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xc4 RCSB], [https://www.ebi.ac.uk/pdbsum/5xc4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xc4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A7KMF0_9CAEN A7KMF0_9CAEN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Although endogenous animal cellulases have great potential for industrial applications such as bioethanol production, few investigations have focused on these enzymes. In this study, the glycoside hydrolase family 45 (GH45) subfamily B endoglucanase EG27II from the snail Ampullaria crossean was expressed using a Pichia pastoris expression system and the crystal structure of the apo form was determined at 1.00 A resolution; this is the highest resolution structure of an animal endoglucanase. The results showed that EG27II has a double-psi six-stranded beta-barrel and that the structure of EG27II more closely resembles those of subfamily C enzymes than those of subfamily A enzymes. The structure of EG27II complexed with cellobiose was also determined under cryoconditions and at room temperature at three pH values, pH 4.0, 5.5 and 8.0, and no structural changes were found to be associated with the change in pH. The structural comparison and catalytic activity measurements showed that Asp137 and Asn112 function as the catalytic acid and base, respectively, and that Asp27 is also an important residue for catalysis. These high-resolution structures of EG27II provide a large amount of information for structure-based enzyme modification and cell-surface engineering, which will advance biofuel production using animal-derived cellulases.

High-resolution crystal structures of the glycoside hydrolase family 45 endoglucanase EG27II from the snail Ampullaria crossean.,Nomura T, Iwase H, Saka N, Takahashi N, Mikami B, Mizutani K Acta Crystallogr D Struct Biol. 2019 Apr 1;75(Pt 4):426-436. doi:, 10.1107/S2059798319003000. Epub 2019 Apr 4. PMID:30988259<ref>PMID:30988259</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xc4" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glucanase 3D structures|Glucanase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Ampullaria crossean]]
[[Category: Large Structures]]
[[Category: Iwase H]]
[[Category: Mikami B]]
[[Category: Mizutani K]]
[[Category: Nomura T]]
[[Category: Takahashi N]]