View source for 5xca


==Crystal structure of GH45 endoglucanase EG27II D137A mutant in complex with cellobiose==
<StructureSection load='5xca' size='340' side='right'caption='[[5xca]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pomacea_maculata Pomacea maculata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XCA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PRD_900005:beta-cellobiose'>PRD_900005</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xca OCA], [https://pdbe.org/5xca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xca RCSB], [https://www.ebi.ac.uk/pdbsum/5xca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xca ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A7KMF0_9CAEN A7KMF0_9CAEN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Although endogenous animal cellulases have great potential for industrial applications such as bioethanol production, few investigations have focused on these enzymes. In this study, the glycoside hydrolase family 45 (GH45) subfamily B endoglucanase EG27II from the snail Ampullaria crossean was expressed using a Pichia pastoris expression system and the crystal structure of the apo form was determined at 1.00 A resolution; this is the highest resolution structure of an animal endoglucanase. The results showed that EG27II has a double-psi six-stranded beta-barrel and that the structure of EG27II more closely resembles those of subfamily C enzymes than those of subfamily A enzymes. The structure of EG27II complexed with cellobiose was also determined under cryoconditions and at room temperature at three pH values, pH 4.0, 5.5 and 8.0, and no structural changes were found to be associated with the change in pH. The structural comparison and catalytic activity measurements showed that Asp137 and Asn112 function as the catalytic acid and base, respectively, and that Asp27 is also an important residue for catalysis. These high-resolution structures of EG27II provide a large amount of information for structure-based enzyme modification and cell-surface engineering, which will advance biofuel production using animal-derived cellulases.

High-resolution crystal structures of the glycoside hydrolase family 45 endoglucanase EG27II from the snail Ampullaria crossean.,Nomura T, Iwase H, Saka N, Takahashi N, Mikami B, Mizutani K Acta Crystallogr D Struct Biol. 2019 Apr 1;75(Pt 4):426-436. doi:, 10.1107/S2059798319003000. Epub 2019 Apr 4. PMID:30988259<ref>PMID:30988259</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xca" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glucanase 3D structures|Glucanase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pomacea maculata]]
[[Category: Iwase H]]
[[Category: Mikami B]]
[[Category: Mizutani K]]
[[Category: Nomura T]]
[[Category: Takahashi N]]