View source for 5xcy


==Structure of the cellobiohydrolase Cel6A from Phanerochaete chrysosporium at 1.2 angstrom==
<StructureSection load='5xcy' size='340' side='right' caption='[[5xcy]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chrysosporium_pruinosum_(gilman_&_abbott)_carmich. Chrysosporium pruinosum (gilman & abbott) carmich.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XCY FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5xcz|5xcz]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cel6A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5306 Chrysosporium pruinosum (Gilman & Abbott) Carmich.])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xcy OCA], [http://pdbe.org/5xcy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xcy RCSB], [http://www.ebi.ac.uk/pdbsum/5xcy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xcy ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cellobiohydrolases belonging to glycoside hydrolase family 6 (CBH II, Cel6A) play key roles in the hydrolysis of crystalline cellulose. CBH II from the white-rot fungus Phanerochaete chrysosporium (PcCel6A) consists of a catalytic domain (CD) and a carbohydrate-binding module connected by a linker peptide, like other known fungal cellobiohydrolases. In the present study, the CD of PcCel6A was crystallized without ligands, and p-nitrophenyl beta-D-cellotrioside (pNPG3) was soaked into the crystals. The determined structures of the ligand-free and pNPG3-soaked crystals revealed that binding of cellobiose at substrate subsites +1 and +2 induces a conformational change of the N-terminal and C-terminal loops, switching the tunnel-shaped active site from the open to the closed form.

Crystal structure of a family 6 cellobiohydrolase from the basidiomycete Phanerochaete chrysosporium.,Tachioka M, Nakamura A, Ishida T, Igarashi K, Samejima M Acta Crystallogr F Struct Biol Commun. 2017 Jul 1;73(Pt 7):398-403. doi:, 10.1107/S2053230X17008093. Epub 2017 Jun 17. PMID:28695848<ref>PMID:28695848</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xcy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Igarashi, K]]
[[Category: Ishida, T]]
[[Category: Nakamura, A]]
[[Category: Samejima, M]]
[[Category: Tachioka, M]]
[[Category: Glycoside hydrolase family 6]]
[[Category: Hydrolase]]