View source for 5xei


==Crystal structure of the Smc head domain with a coiled coil and joint derived from Pyrococcus yayanosii==
<StructureSection load='5xei' size='340' side='right'caption='[[5xei]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xei]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_yayanosii_CH1 Pyrococcus yayanosii CH1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XEI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XEI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.599&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xei OCA], [https://pdbe.org/5xei PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xei RCSB], [https://www.ebi.ac.uk/pdbsum/5xei PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xei ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F8AFS8_PYRYC F8AFS8_PYRYC] Required for chromosome condensation and partitioning.[HAMAP-Rule:MF_01894]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase "head" and a "hinge" dimerization domain connected by a 49 nm coiled-coil "arm." The heads undergo ATP-dependent engagement and disengagement to drive SMC action on the chromosome. Here, we elucidate the architecture of prokaryotic Smc dimers by high-throughput cysteine cross-linking and crystallography. Co-alignment of the Smc arms tightly closes the interarm space and misaligns the Smc head domains at the end of the rod by close apposition of their ABC signature motifs. Sandwiching of ATP molecules between Smc heads requires them to substantially tilt and translate relative to each other, thereby opening up the Smc arms. We show that this mechanochemical gating reaction regulates chromosome targeting and propose a mechanism for DNA translocation based on the merging of DNA loops upon closure of Smc arms.

Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization.,Diebold-Durand ML, Lee H, Ruiz Avila LB, Noh H, Shin HC, Im H, Bock FP, Burmann F, Durand A, Basfeld A, Ham S, Basquin J, Oh BH, Gruber S Mol Cell. 2017 Jul 20;67(2):334-347.e5. doi: 10.1016/j.molcel.2017.06.010. Epub, 2017 Jul 6. PMID:28689660<ref>PMID:28689660</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xei" style="background-color:#fffaf0;"></div>

==See Also==
*[[Condensin|Condensin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pyrococcus yayanosii CH1]]
[[Category: Lee H]]
[[Category: Noh H]]
[[Category: Oh B-H]]