View source for 5xgr


==Structure of the S1 subunit C-terminal domain from bat-derived coronavirus HKU5 spike protein==
<StructureSection load='5xgr' size='340' side='right'caption='[[5xgr]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xgr]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pipistrellus_bat_coronavirus_HKU5 Pipistrellus bat coronavirus HKU5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XGR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XGR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xgr OCA], [https://pdbe.org/5xgr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xgr RCSB], [https://www.ebi.ac.uk/pdbsum/5xgr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xgr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPIKE_BCHK5 SPIKE_BCHK5] S1 region attaches the virion to the cell membrane by interacting with cell receptors, initiating the infection.  S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes (By similarity).
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== Publication Abstract from PubMed ==
Accumulating evidence indicates that MERS-CoV originated from bat coronaviruses (BatCoVs). Previously, we demonstrated that both MERS-CoV and BatCoV HKU4 use CD26 as a receptor, but how the BatCoVs evolved to bind CD26 is an intriguing question. Here, we solved the crystal structure of the S1 subunit C-terminal domain of HKU5 (HKU5-CTD), another BatCoV that is phylogenetically related to MERS-CoV but cannot bind to CD26. We observed that the conserved core subdomain and those of other betacoronaviruses (betaCoVs) have a similar topology of the external subdomain, indicating the same ancestor of lineage C betaCoVs. However, two deletions in two respective loops located in HKU5-CTD result in conformational variations in CD26-binding interface and are responsible for the non-binding of HKU5-CTD to CD26. Combined with sequence variation in the HKU5-CTD receptor binding interface, we propose the necessity for surveilling the mutation in BatCoV HKU5 spike protein in case of bat-to-human interspecies transmission.

Structure of the S1 subunit C-terminal domain from bat-derived coronavirus HKU5 spike protein.,Han X, Qi J, Song H, Wang Q, Zhang Y, Wu Y, Lu G, Yuen KY, Shi Y, Gao GF Virology. 2017 Apr 19;507:101-109. doi: 10.1016/j.virol.2017.04.016. PMID:28432925<ref>PMID:28432925</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5xgr" style="background-color:#fffaf0;"></div>

==See Also==
*[[Sandbox 3001|Sandbox 3001]]
*[[Spike protein 3D structures|Spike protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pipistrellus bat coronavirus HKU5]]
[[Category: Gao GF]]
[[Category: Qi J]]
[[Category: Qihui W]]
[[Category: Shi Y]]
[[Category: Song H]]
[[Category: Xue H]]