View source for 5xhb


==Crystal structure of the full length of NisI in a lipid free form, the nisin immunity protein, from Lactococcus lactis==
<StructureSection load='5xhb' size='340' side='right' caption='[[5xhb]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xhb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"lactococcus_lactis_subsp._diacitilactis_(sic)"_(garvie_and_farrow_1982)_schleifer_et_al._1986 "lactococcus lactis subsp. diacitilactis (sic)" (garvie and farrow 1982) schleifer et al. 1986]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XHB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XHB FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nisI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1360 "Lactococcus lactis subsp. diacitilactis (sic)" (Garvie and Farrow 1982) Schleifer et al. 1986])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xhb OCA], [http://pdbe.org/5xhb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xhb RCSB], [http://www.ebi.ac.uk/pdbsum/5xhb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xhb ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/NISI_LACLL NISI_LACLL]] Involved in immunity against exogenously supplied nisin. 
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== Publication Abstract from PubMed ==
Nisin is a lantibiotic, a family of polypeptides containing lanthionine with antimicrobial activity. Nisin-producing microorganisms require immunity proteins for self-protection from nisin itself. Lactococcus lactis, a microorganism that synthesizes nisin, has an integral NisFEG ABC transporter and NisI lipoprotein that function in the nisin immunity. Here, we present the crystal structure of the full length of NisI22-C, a lipid-free form of NisI, determined at 1.9 A resolution. Similar to the NMR structures of N- and C-terminal domain of NisI, NisI22-C is composed of N- and C-terminal domains, both of which display a fold similar to that found in SpaI, a lipoprotein with immunity against subtilin in Bacillus subtilis The full length structure of NisI22-c reveals a large and deep cleft by the interdomain association, one side of which is occupied by the residues important for immunity. Opposite to the cleft, a shallow groove is found where nisin-interacting residues are distributed in the periphery composed of the C-terminal negative patch. Based on a sulfate ion found in the large and deep cleft, a model of NisI in complex with a farnesyl diphosphate backbone of lipid II is proposed, suggesting a mechanism for increasing the chances of encountering with nisin.

Crystal Structure of NisI in a Lipid Free Form, the Nisin Immunity Protein, from Lactococcus lactis.,Jeong JH, Ha SC Antimicrob Agents Chemother. 2018 Jan 8. pii: AAC.01966-17. doi:, 10.1128/AAC.01966-17. PMID:29311076<ref>PMID:29311076</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5xhb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Ha, S C]]
[[Category: Immune system]]
[[Category: Immunity protein]]