View source for 5xkb


==Crystal Structure of HasAp with Fe-5,15-bisethynyl-10,20-diphenylporphyrin==
<StructureSection load='5xkb' size='340' side='right'caption='[[5xkb]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xkb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XKB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XKB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xkb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xkb OCA], [https://pdbe.org/5xkb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xkb RCSB], [https://www.ebi.ac.uk/pdbsum/5xkb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xkb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G3XD33_PSEAE G3XD33_PSEAE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Iron(III)-5,15-diphenylporphyrin and several derivatives were accommodated by HasA, a heme acquisition protein secreted by Pseudomonas aeruginosa, despite possessing bulky substituents at the meso position of the porphyrin. Crystal structure analysis revealed that the two phenyl groups at the meso positions of porphyrin extend outside HasA. It was shown that the growth of P. aeruginosa was inhibited in the presence of HasA coordinating the synthetic porphyrins under iron-limiting conditions, and that the structure of the synthetic porphyrins greatly affects the inhibition efficiency.

Structures of the Heme Acquisition Protein HasA with Iron(III)-5,15-Diphenylporphyrin and Derivatives Thereof as an Artificial Prosthetic Group.,Uehara H, Shisaka Y, Nishimura T, Sugimoto H, Shiro Y, Miyake Y, Shinokubo H, Watanabe Y, Shoji O Angew Chem Int Ed Engl. 2017 Nov 27;56(48):15279-15283. doi:, 10.1002/anie.201707212. Epub 2017 Oct 9. PMID:28921809<ref>PMID:28921809</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xkb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Shiro Y]]
[[Category: Shoji O]]
[[Category: Sugimoto H]]
[[Category: Uehara H]]
[[Category: Watanabe Y]]