View source for 5xkj


==Crystal structure of plant receptor ERL1-TMM in complexe with EPF2==
<StructureSection load='5xkj' size='340' side='right'caption='[[5xkj]], [[Resolution|resolution]] 3.48&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xkj]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XKJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XKJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.475&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xkj OCA], [https://pdbe.org/5xkj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xkj RCSB], [https://www.ebi.ac.uk/pdbsum/5xkj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xkj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TMM_ARATH TMM_ARATH] Promotes cell fate progression in stomatal development. In leaves, needed to correctly orient spacing divisions, to limit the number of asymmetric divisions in neighbor cells, and to promote the asymmetric (amplifying) divisions of meristemoids. In stems, promotes the conversion of meristemoids into guard mother cells (GMC) (PubMed:11090210, PubMed:12040198, PubMed:18979118). Positively regulates CAPRICE (CPC) expression in differentiating stomaless-forming cell files (PubMed:19513241). Forms constitutive complexes with ERECTA and ERL1 involved in the recognition of the stomatal regulatory peptides EPF1, EPF2 and EPFL9/STOMAGEN (PubMed:28536146). Modulates the activity of the ligand-receptor pairs EPF2-ERECTA and EPF1-ERL1 in stomatal development (PubMed:16002616, PubMed:22241782). Functions in a combinatorial specific manner with the ERECTA-family (ERf) receptor kinases in the regulation of the immune response (PubMed:27446127).<ref>PMID:11090210</ref> <ref>PMID:12040198</ref> <ref>PMID:16002616</ref> <ref>PMID:18979118</ref> <ref>PMID:19513241</ref> <ref>PMID:22241782</ref> <ref>PMID:27446127</ref> <ref>PMID:28536146</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Stomata are microscopic openings that allow for the exchange of gases between plants and the environment. In Arabidopsis, stomatal patterning is specified by the ERECTA family (ERf) receptor kinases (RKs), the receptor-like protein (RLP) TOO MANY MOUTHS (TMM), and EPIDERMAL PATTERNING FACTOR (EPF) peptides. Here we show that TMM and ER or ER-LIKE1 (ERL1) form constitutive complexes, which recognize EPF1 and EPF2, but the single ERfs do not. TMM interaction with ERL1 creates a binding pocket for recognition of EPF1 and EPF2, indicating that the constitutive TMM-ERf complexes function as the receptors of EPF1 and EPF2. EPFL9 competes with EPF1 and EPF2 for binding to the ERf-TMM complex. EPFL4 and EPFL6, however, are recognized by the single ERfs without the requirement of TMM. In contrast to EPF1,2, the interaction of EPFL4,6 with an ERf is greatly reduced in the presence of TMM. Taken together, our data demonstrate that TMM dictates the specificity of ERfs for the perception of different EPFs, thus functioning as a specificity switch for the regulation of the activities of ERfs.

A receptor-like protein acts as a specificity switch for the regulation of stomatal development.,Lin G, Zhang L, Han Z, Yang X, Liu W, Li E, Chang J, Qi Y, Shpak ED, Chai J Genes Dev. 2017 May 1;31(9):927-938. doi: 10.1101/gad.297580.117. Epub 2017 May, 23. PMID:28536146<ref>PMID:28536146</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
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== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Chai J]]
[[Category: Lin G]]