View source for 5xmz


==Verticillium effector PevD1==
<StructureSection load='5xmz' size='340' side='right'caption='[[5xmz]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xmz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Verticillium_dahliae Verticillium dahliae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XMZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XMZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xmz OCA], [https://pdbe.org/5xmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xmz RCSB], [https://www.ebi.ac.uk/pdbsum/5xmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xmz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PEVD1_VERDA PEVD1_VERDA] Effector protein (PubMed:21691787, PubMed:24080193, PubMed:24337817). Elicits a hypersensitive response (HR) in tobacco plants (N.tabacum) and cotton (G.hirsutum) (PubMed:21691787, PubMed:24080193, PubMed:24337817). Boosts systemic acquired resistance (SAR) to tobacco mosaic virus (TMV) infection in N.tabacum and to V.dhaliae infection in primed cotton seedlings (PubMed:21691787, PubMed:24080193, PubMed:24337817).<ref>PMID:21691787</ref> <ref>PMID:24080193</ref> <ref>PMID:24337817</ref> 
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== Publication Abstract from PubMed ==
The soil-borne fungal pathogen Verticillium dahliae infects a wide range of dicotyledonous plants including cotton, tobacco, and Arabidopsis. Among the effector proteins secreted by V. dahliae, the 16 kDa PevD1 induces a hypersensitive response in tobacco. Here we report the high-resolution structure of PevD1 with folds resembling a C2 domain-like structure with a calcium ion bound to the C-terminal acidic pocket. A yeast two-hybrid screen, designed to probe for molecular functions of PevD1, identified Arabidopsis asparagine-rich protein (NRP) as the interacting partner of PevD1. Extending the pathway of V. dahliae effects, which include induction of early flowering in cotton and Arabidopsis, NRP was found to interact with cryptochrome 2 (CRY2), leading to increased cytoplasmic accumulation of CRY2 in a blue light-independent manner. Further physiological and genetic evidence suggests that PevD1 indirectly activates CRY2 by antagonizing NRP functions. The promotion of CRY2-mediated flowering by a fungal effector outlines a novel pathway by which an external stimulus is recognized and transferred in changing a developmental program.

The asparagine-rich protein NRP interacts with the Verticillium effector PevD1 and regulates the subcellular localization of cryptochrome 2.,Zhou R, Zhu T, Han L, Liu M, Xu M, Liu Y, Han D, Qiu D, Gong Q, Liu X J Exp Bot. 2017 Jun 15;68(13):3427-3440. doi: 10.1093/jxb/erx192. PMID:28633330<ref>PMID:28633330</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5xmz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Verticillium dahliae]]
[[Category: Liu X]]
[[Category: Zhou R]]