View source for 5xo1


==Crystal structure of the isochorismatase domain of VabB from Vibrio anguillarum 775==
<StructureSection load='5xo1' size='340' side='right'caption='[[5xo1]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xo1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_anguillarum_775 Vibrio anguillarum 775]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XO1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XO1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xo1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xo1 OCA], [https://pdbe.org/5xo1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xo1 RCSB], [https://www.ebi.ac.uk/pdbsum/5xo1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xo1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q5DK16_VIBA7 Q5DK16_VIBA7] 
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== Publication Abstract from PubMed ==
Antibiotic resistance is becoming a global threat and overuse of antibiotics in aquaculture disease control worsens the situation. To reduce the risk of drug resistance developed in aquaculture, safer biocontrol programs are needed. Antivirulence therapy, with less chance for developing drug resistance, is a promising approach. To facilitate antivirulence inhibitor design against Vibrio anguillarum, a serious aquaculture pathogen, we present crystal structures for isochorismatase domains of AngB and VabB, which are required to synthesize siderophore, a critical virulence factor. Both structures are highly similar to known isochorismatases in fold and active site, therefore we conclude inhibitors for isochorismatases can be developed in a common framework. The structural information will improve design of virulence inhibitors against Vibrio anguillarum. We also firstly report that isochorismatase family could bind endogenous metabolite during the hetero-expression process, which is likely nicotinic acid, nicotinamide or pyrazinic acid, based on structural analysis and affinity prediction. Taken together, our results provide precise structural information of isochorismatase domains for antivirulence inhibitor design against Vibrio anguillarum.

Crystal structures of the isochorismatase domains from Vibrio anguillarum.,Du J, Deng T, Ma Q Biochem Biophys Res Commun. 2017 Aug 26;490(3):827-833. doi:, 10.1016/j.bbrc.2017.06.125. Epub 2017 Jun 21. PMID:28647364<ref>PMID:28647364</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xo1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio anguillarum 775]]
[[Category: Du J]]
[[Category: Ma Q]]