View source for 5xoj


==Crystal structure of Xpo1p-PKI-Nup42p-Gsp1p-GTP complex==
<StructureSection load='5xoj' size='340' side='right'caption='[[5xoj]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xoj]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_AWRI796 Saccharomyces cerevisiae AWRI796], [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_FostersB Saccharomyces cerevisiae FostersB] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XOJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xoj OCA], [https://pdbe.org/5xoj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xoj RCSB], [https://www.ebi.ac.uk/pdbsum/5xoj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xoj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/XPO1_YEAST XPO1_YEAST] Receptor for the leucine-rich nuclear export signal (NES).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Xpo1p (yeast CRM1) is the major nuclear export receptor that carries a plethora of proteins and ribonucleoproteins from the nucleus to cytoplasm. The passage of the Xpo1p nuclear export complex through nuclear pore complexes (NPCs) is facilitated by interactions with nucleoporins (Nups) containing extensive repeats of phenylalanine-glycine (so-called FG repeats), although the precise role of each Nup in the nuclear export reaction remains incompletely understood. Here we report structural and biochemical characterization of the interactions between the Xpo1p nuclear export complex and the FG repeats of Nup42p, a nucleoporin localized at the cytoplasmic face of yeast NPCs and has characteristic SxFG/PxFG sequence repeat motif. The crystal structure of Xpo1p-PKI-Nup42p-Gsp1p-GTP complex identified three binding sites for the SxFG/PxFG repeats on HEAT repeats 14-20 of Xpo1p. Mutational analyses of Nup42p showed that the conserved serines and prolines in the SxFG/PxFG repeats contribute to Xpo1p-Nup42p binding. Our structural and biochemical data suggest that SxFG/PxFG-Nups such as Nup42p and Nup159p at the cytoplasmic face of NPCs provide high-affinity docking sites for the Xpo1p nuclear export complex in the terminal stage of NPC passage and that subsequent disassembly of the nuclear export complex facilitates recycling of free Xpo1p back to the nucleus.

Crystal structure of the Xpo1p nuclear export complex bound to the SxFG/PxFG repeats of the nucleoporin Nup42p.,Koyama M, Hirano H, Shirai N, Matsuura Y Genes Cells. 2017 Oct;22(10):861-875. doi: 10.1111/gtc.12520. Epub 2017 Aug 8. PMID:28791779<ref>PMID:28791779</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xoj" style="background-color:#fffaf0;"></div>

==See Also==
*[[Exportin 3D structures|Exportin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae AWRI796]]
[[Category: Saccharomyces cerevisiae FostersB]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Koyama M]]
[[Category: Matsuura Y]]
[[Category: Shirai N]]