View source for 5xua


==The ligand-free dimer of chemoreceptor MCP2201 ligand binding domain==
<StructureSection load='5xua' size='340' side='right'caption='[[5xua]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xua]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Comamonas_testosteroni_CNB-2 Comamonas testosteroni CNB-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XUA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xua OCA], [https://pdbe.org/5xua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xua RCSB], [https://www.ebi.ac.uk/pdbsum/5xua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xua ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D0IVL9_COMT2 D0IVL9_COMT2] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Transmembrane chemoreceptors are widely present in Bacteria and Archaea. They play a critical role in sensing various signals outside and transmitting to the cell interior. Here, we report the structure of the periplasmic ligand-binding domain (LBD) of the transmembrane chemoreceptor MCP2201, which governs chemotaxis to citrate and other organic compounds in Comamonas testosteroni. The apo-form LBD crystal revealed a typical four-helix bundle homodimer, similar to previously well-studied chemoreceptors such as Tar and Tsr of Escherichia coli. However, the citrate-bound LBD revealed a four-helix bundle homotrimer that had not been observed in bacterial chemoreceptor LBDs. This homotrimer was further confirmed with size-exclusion chromatography, analytical ultracentrifugation and cross-linking experiments. The physiological importance of the homotrimer for chemotaxis was demonstrated with site-directed mutations of key amino acid residues in C. testosteroni mutants.

The ligand-binding domain of a chemoreceptor from Comamonas testosteroni has a previously unknown homotrimeric structure.,Hong Y, Huang Z, Guo L, Ni B, Jiang CY, Li XJ, Hou YJ, Yang WS, Wang DC, Zhulin IB, Liu SJ, Li DF Mol Microbiol. 2019 Jun 8. doi: 10.1111/mmi.14326. PMID:31177588<ref>PMID:31177588</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xua" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Comamonas testosteroni CNB-2]]
[[Category: Large Structures]]
[[Category: Hong Y]]
[[Category: Li DF]]
[[Category: Wang DC]]