View source for 5xwp


==Crystal structure of LbuCas13a-crRNA-target RNA ternary complex==
<StructureSection load='5xwp' size='340' side='right'caption='[[5xwp]], [[Resolution|resolution]] 3.09&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5xwp]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XWP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XWP FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xwp OCA], [http://pdbe.org/5xwp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xwp RCSB], [http://www.ebi.ac.uk/pdbsum/5xwp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xwp ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Cas13a, a type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, degrades invasive RNAs targeted by CRISPR RNA (crRNA) and has potential applications in RNA technology. To understand how Cas13a is activated to cleave RNA, we have determined the crystal structure of Leptotrichia buccalis (Lbu) Cas13a bound to crRNA and its target RNA, as well as the cryo-EM structure of the LbuCas13a-crRNA complex. The crRNA-target RNA duplex binds in a positively charged central channel of the nuclease (NUC) lobe, and Cas13a protein and crRNA undergo a significant conformational change upon target RNA binding. The guide-target RNA duplex formation triggers HEPN1 domain to move toward HEPN2 domain, activating the HEPN catalytic site of Cas13a protein, which subsequently cleaves both single-stranded target and collateral RNAs in a non-specific manner. These findings reveal how Cas13a of type VI CRISPR-Cas systems defend against RNA phages and set the stage for its development as a tool for RNA manipulation.

The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a.,Liu L, Li X, Ma J, Li Z, You L, Wang J, Wang M, Zhang X, Wang Y Cell. 2017 Aug 10;170(4):714-726.e10. doi: 10.1016/j.cell.2017.06.050. Epub 2017 , Jul 27. PMID:28757251<ref>PMID:28757251</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5xwp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Li, X]]
[[Category: Li, Z]]
[[Category: Liu, L]]
[[Category: Wang, Y]]
[[Category: C2c2]]
[[Category: Crrna]]
[[Category: Lbucas13a]]
[[Category: Rna binding protein-rna complex]]
[[Category: Target rna]]