View source for 5y36


==Cryo-EM structure of SpCas9-sgRNA-DNA ternary complex==
<SX load='5y36' size='340' side='right' viewer='molstar' caption='[[5y36]], [[Resolution|resolution]] 5.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5y36]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_serotype_M1 Streptococcus pyogenes serotype M1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y36 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Y36 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5y36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y36 OCA], [https://pdbe.org/5y36 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5y36 RCSB], [https://www.ebi.ac.uk/pdbsum/5y36 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5y36 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CAS9_STRP1 CAS9_STRP1] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.<ref>PMID:21455174</ref> <ref>PMID:22745249</ref> 

==See Also==
*[[CRISPR-Cas9|CRISPR-Cas9]]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
<references/>
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</SX>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Streptococcus pyogenes serotype M1]]
[[Category: Huai C]]
[[Category: Huang Q]]
[[Category: Li G]]