View source for 5yjs


==Structure of vicilin from Capsicum annuum==
<StructureSection load='5yjs' size='340' side='right'caption='[[5yjs]], [[Resolution|resolution]] 2.16&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5yjs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Capsicum_annuum Capsicum annuum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YJS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YJS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.16&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yjs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yjs OCA], [https://pdbe.org/5yjs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yjs RCSB], [https://www.ebi.ac.uk/pdbsum/5yjs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yjs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A452CSM5_CAPAN A0A452CSM5_CAPAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Proteins belonging to cupin superfamily are known to have critical and diverse physiological functions. However, 7S globulins family, which is also a part of cupin superfamily were undermined as only seed storage proteins. Structure determination of native protein - Vic_CAPAN from Capsicumannuum was carried out, and its physiological functions were explored after purifying the protein by ammonium sulfate precipitation followed by size exclusion chromatography. The crystal structure of vicilin determined at 2.16 A resolution revealed two monomers per asymmetric unit which are juxtaposed orthogonal with each other. Vic_CAPAN consists predominately of beta-sheets which folds to form a beta-barrel structure commonly called cupin fold. Each monomer of Vic_CAPAN consists of two cupin fold domains, N-terminal and C-terminal, which accommodate two different ligands. A bound ligand was identified at the C-terminal cupin fold in the site presumably conserved for metabolites in the crystal structure. The ligand was confirmed to be salicylic acid through mass spectrometric analysis. A copper binding site was further observed near the conserved ligand binding pocket, suggesting possible superoxide dismutase activity of Vic_CAPAN which was subsequently confirmed biochemically. Vicilins from other sources did not exhibit this activity indicating functional specificity of Vic_CAPAN. Discovery of bound salicylic acid, which is a known regulator of antioxidant pathway and revelation of superoxide dismutase activity suggest that Vic_CAPAN has important role during oxidative stress. As salicylic acid changes the redox state of cell, it may act as a downstream signal for various pathways involved in plant biotic and abiotic stress rescue.

Structure-guided identification of function: role of C. annuum vicilin during oxidative stress.,Shikhi M, Nair DT, Salunke DM Biochem J. 2018 Sep 4. pii: BCJ20180520. doi: 10.1042/BCJ20180520. PMID:30181145<ref>PMID:30181145</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5yjs" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Capsicum annuum]]
[[Category: Large Structures]]
[[Category: Nair DT]]
[[Category: Salunke DM]]
[[Category: Shikhi M]]