View source for 5yow


==The post-fusion structure of the Heartland virus Gc glycoprotein==
<StructureSection load='5yow' size='340' side='right'caption='[[5yow]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5yow]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Heartland_virus Heartland virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YOW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yow OCA], [https://pdbe.org/5yow PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yow RCSB], [https://www.ebi.ac.uk/pdbsum/5yow PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yow ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GP_HTRV GP_HTRV] Structural component of the virion that interacts with glycoprotein C (By similarity). It shields the hydrophobic fusion loops of the glycoprotein C, preventing premature fusion (By similarity). The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation (By similarity). They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after endocytosis of the virion (Probable). Plays a role in the packaging of ribonucleoproteins during virus assembly (By similarity).[UniProtKB:P03518][UniProtKB:P09613][UniProtKB:P21401]<ref>PMID:33561699</ref>   Structural component of the virion that interacts with glycoprotein N (By similarity). Acts as a class II fusion protein that is activated upon acidification and subsequent repositioning of the glycoprotein N (PubMed:29070692). The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation (By similarity). They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after endocytosis of the virion (Probable).[UniProtKB:P03518][UniProtKB:P09613]<ref>PMID:29070692</ref> <ref>PMID:33561699</ref> 
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== Publication Abstract from PubMed ==
Heartland virus (HRTV) is an emerging human pathogen that belongs to the newly defined family Phenuiviridae, order Bunyavirales Gn and Gc are two viral surface glycoproteins encoded by the M segment and are required for early events during infection. HRTV delivers its genome into the cytoplasm by fusion of the viral envelope and endosomal membranes under low pH conditions. Here, we describe the crystal structure of HRTV Gc in its post-fusion conformation. The structure shows that Gc displays a typical class II fusion protein conformation, and the overall structure is identical to severe fever with thrombocytopenia syndrome virus (SFTSV) Gc, which also belongs to the Phenuiviridae family. However, our structural analysis indicates that the hantavirus Gc presents distinct feature in the aspects of subdomain orientation, N-linked glycosylation, the interactions pattern between protomers, and the fusion loop conformation. This suggests their family-specific subunit arrangement during the fusogenic process and supports the recent taxonomic revision of bunyaviruses. Our results provide insights into the comprehensive comparison of class II membrane fusion proteins in two bunyavirus families, yielding valuable information for treatments against these human pathogens.IMPORTANCE HRTV is an insect-borne virus found in America that can infect humans. It belongs to the newly defined family Phenuiviridae, order Bunyavirales HRTV contains three single-stranded RNA segments (L, M, and S). The M segment of the virus encodes a polyprotein precursor that is cleaved into two glycoproteins, Gn and Gc. Gc is a fusion protein facilitating virus entry into host cells. Here, we report the crystal structure of the HRTV Gc protein. The structure displays a typical class II fusion protein conformation. Comparison of HRTV Gc with a recently solved structure of another bunyavirus Gc revealed that these Gc structures display a newly defined family specificity, supporting the recent International Committee of Taxonomy of Viruses re-classification of the bunyaviruses. Our results expand the knowledge of bunyavirus fusion proteins and help us to understand bunyavirus characterizations. This study provides useful information to improve protection against and therapies for bunyavirus infections.

The post-fusion structure of the Heartland virus Gc glycoprotein supports taxonomic separation of the bunyaviral families Phenuiviridae and Hantaviridae.,Zhu Y, Wu Y, Chai Y, Qi J, Peng R, Feng WH, Gao GF J Virol. 2017 Oct 25. pii: JVI.01558-17. doi: 10.1128/JVI.01558-17. PMID:29070692<ref>PMID:29070692</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Heartland virus]]
[[Category: Large Structures]]
[[Category: Chai Y]]
[[Category: Gao F]]
[[Category: Qi JX]]
[[Category: Wu Y]]