View source for 5yq0


==Crystal structure of secreted protein CofJ from ETEC.==
<StructureSection load='5yq0' size='340' side='right' caption='[[5yq0]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5yq0]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YQ0 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cofJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yq0 OCA], [http://pdbe.org/5yq0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yq0 RCSB], [http://www.ebi.ac.uk/pdbsum/5yq0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yq0 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Initial attachment and subsequent colonization of the intestinal epithelium comprise critical events allowing enteric pathogens to survive and express their pathogenesis. In enterotoxigenic Escherichia coli (ETEC), these are mediated by a long proteinaceous fiber termed type IVb pilus (T4bP). We have reported that the colonization factor antigen/III (CFA/III), an operon-encoded T4bP of ETEC, possesses a minor pilin, CofB, that carries an H-type lectin domain at its tip. Although CofB is critical for pilus assembly by forming a trimeric initiator complex, its importance for bacterial attachment remains undefined. Here, we show that T4bP is not sufficient for bacterial attachment, which also requires a secreted protein CofJ, encoded within the same CFA/III operon. The crystal structure of CofB complexed with a peptide encompassing the binding region of CofJ showed that CofJ interacts with CofB by anchoring its flexible N-terminal extension to be embedded deeply into the expected carbohydrate recognition site of the CofB H-type lectin domain. By combining this structure and physicochemical data in solution, we built a plausible model of the CofJ-CFA/III pilus complex, which suggested that CofJ acts as a molecular bridge by binding both T4bP and the host cell membrane. The Fab fragments of a polyclonal antibody against CofJ significantly inhibited bacterial attachment by preventing the adherence of secreted CofJ proteins. These findings signify the interplay between T4bP and a secreted protein for attaching to and colonizing the host cell surface, potentially constituting a therapeutic target against ETEC infection.

Interplay of a secreted protein with type IVb pilus for efficient enterotoxigenic Escherichia coli colonization.,Oki H, Kawahara K, Maruno T, Imai T, Muroga Y, Fukakusa S, Iwashita T, Kobayashi Y, Matsuda S, Kodama T, Iida T, Yoshida T, Ohkubo T, Nakamura S Proc Natl Acad Sci U S A. 2018 Jul 10;115(28):7422-7427. doi:, 10.1073/pnas.1805671115. Epub 2018 Jun 25. PMID:29941571<ref>PMID:29941571</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5yq0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus coli migula 1895]]
[[Category: Fukakusa, S]]
[[Category: Iida, T]]
[[Category: Imai, T]]
[[Category: Iwashita, T]]
[[Category: Kawahara, K]]
[[Category: Kobayashi, Y]]
[[Category: Kodama, T]]
[[Category: Maruno, T]]
[[Category: Matsuda, S]]
[[Category: Muroga, Y]]
[[Category: Nakamura, S]]
[[Category: Ohkubo, T]]
[[Category: Oki, H]]
[[Category: Yoshida, T]]
[[Category: Cell adhesion]]
[[Category: Cfa/iii]]
[[Category: Colonization factor]]
[[Category: Type ivb pili]]