View source for 5ys2


==Structure of the domain IV(D_IV) of Pseudorabies virus glycoprotein B( PRV gB)==
<StructureSection load='5ys2' size='340' side='right'caption='[[5ys2]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ys2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Suid_alphaherpesvirus_1 Suid alphaherpesvirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YS2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.698&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ys2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ys2 OCA], [https://pdbe.org/5ys2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ys2 RCSB], [https://www.ebi.ac.uk/pdbsum/5ys2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ys2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T2FL65_SUHV T2FL65_SUHV] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pseudorabies virus (PRV) belongs to the Herpesviridae family, and is an important veterinary pathogen. Highly pathogenic PRV variants have caused severe epidemics in China since 2011, causing huge economic losses. To tackle the epidemics, we identified a panel of mouse monoclonal antibodies (mAbs) against PRV glycoprotein B (gB) that effectively block PRV infection. Among these 15 mAbs, fourteen of them block PRV entry in a complement-dependent manner. The remaining one, 1H1 mAb, however can directly neutralize the virus independent of complement and displays broad-spectrum neutralizing activities. We further determined the crystal structure of PRV gB and mapped the epitopes of these antibodies on the structure. Interestingly, all the complement-dependent neutralizing antibodies bind gB at the crown region (domain IV). In contrast, the epitope of 1H1 mAb is located at the bottom of domain I, which includes the fusion loops, indicating 1H1 mAb might neutralize the virus by interfering with the membrane fusion process. Our studies demonstrate that gB contains multiple B-cell epitopes in its crown and base regions and that antibodies targeting different epitopes block virus infection through different mechanisms. These findings would provide important clues for antiviral drug design and vaccine development.

Two classes of protective antibodies against Pseudorabies virus variant glycoprotein B: Implications for vaccine design.,Li X, Yang F, Hu X, Tan F, Qi J, Peng R, Wang M, Chai Y, Hao L, Deng J, Bai C, Wang J, Song H, Tan S, Lu G, Gao GF, Shi Y, Tian K PLoS Pathog. 2017 Dec 20;13(12):e1006777. doi: 10.1371/journal.ppat.1006777., eCollection 2017 Dec. PMID:29261802<ref>PMID:29261802</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ys2" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glycoproteins B and D|Glycoproteins B and D]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Suid alphaherpesvirus 1]]
[[Category: Hu XL]]
[[Category: Yang FL]]