View source for 5yzw


==Crystal structure of p204 HINb domain==
<StructureSection load='5yzw' size='340' side='right'caption='[[5yzw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5yzw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus_domesticus Mus musculus domesticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YZW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.001&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yzw OCA], [https://pdbe.org/5yzw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yzw RCSB], [https://www.ebi.ac.uk/pdbsum/5yzw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yzw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IFI4_MOUSE IFI4_MOUSE] Inhibits the transcription of ribosomal RNA. May inhibit DNA binding by UBTF. Inhibits cell growth via p53/TP53 and RB1-dependent and independent pathways. Acts as a coactivator of RUNX2 during osteogenesis. May be involved in macrophage differentiation. Enables skeletal muscle and cardiac myocyte differentiation by sequestring Id proteins in the cytosol and promoting their ubiquitination and subsequent degradation.<ref>PMID:10329630</ref> <ref>PMID:11940648</ref> <ref>PMID:15557274</ref> <ref>PMID:16244109</ref> <ref>PMID:16458891</ref> <ref>PMID:16556595</ref> <ref>PMID:16556596</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The interferon gamma-inducible protein 16 (IFI16) and its murine homologous protein p204 function in non-sequence specific dsDNA sensing; however, the exact dsDNA recognition mechanisms of IFI16/p204, which harbour two HIN domains, remain unclear. In the present study, we determined crystal structures of p204 HINa and HINb domains, which are highly similar to those of other PYHIN family proteins. Moreover, we obtained the crystal structure of p204 HINab domain in complex with dsDNA and provided insights into the dsDNA binding mode. p204 HINab binds dsDNA mainly through alpha2 helix of HINa and HINb, and the linker between them, revealing a similar HIN:DNA binding mode. Both HINa and HINb are vital for HINab recognition of dsDNA, as confirmed by fluorescence polarization assays. Furthermore, a HINa dimerization interface was observed in structures of p204 HINa and HINab:dsDNA complex, which is involved in binding dsDNA. The linker between HINa and HINb reveals dynamic flexibility in solution and changes its direction at approximately 90 degrees angle in comparison with crystal structure of HINab:dsDNA complex. These structural information provide insights into the mechanism of DNA recognition by different HIN domains, and shed light on the unique roles of two HIN domains in activating the IFI16/p204 signaling pathway.

Structural mechanism of DNA recognition by the p204 HIN domain.,Fan X, Jiang J, Zhao D, Chen F, Ma H, Smith P, Unterholzner L, Xiao TS, Jin T Nucleic Acids Res. 2021 Feb 22. pii: 6146633. doi: 10.1093/nar/gkab076. PMID:33619523<ref>PMID:33619523</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5yzw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus domesticus]]
[[Category: Jin T]]