View source for 5zcn


==Solution NMR structure of a new lasso peptide brevunsin==
<StructureSection load='5zcn' size='340' side='right'caption='[[5zcn]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5zcn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZCN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZCN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 15 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zcn OCA], [https://pdbe.org/5zcn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zcn RCSB], [https://www.ebi.ac.uk/pdbsum/5zcn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zcn ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was successfully produced by S. subterranea, harboring the expression vector, with a high production yield (10.2 mg from 1 L culture). The chemical structure of brevunsin was established by NMR and MS/MS experiments. Based on the information obtained from the NOE experiment, the three-dimensional structure of brevunsin was determined, which indicated that brevunsin possessed a typical lasso structure. This expression vector system provides a new heterologous production method for unexplored lasso peptides that are encoded by bacterial genomes.

Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea.,Kodani S, Hemmi H, Miyake Y, Kaweewan I, Nakagawa H J Ind Microbiol Biotechnol. 2018 Nov;45(11):983-992. doi:, 10.1007/s10295-018-2077-6. Epub 2018 Sep 6. PMID:30191430<ref>PMID:30191430</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5zcn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Brevundimonas diminuta]]
[[Category: Large Structures]]
[[Category: Hemmi H]]
[[Category: Kaweewan I]]
[[Category: Kodani S]]
[[Category: Miyake Y]]
[[Category: Nakagawa H]]