View source for 5zef


==Crystal structure of Entamoeba histolytica Arginase in complex with L- Norvaline at 2.01 A==
<StructureSection load='5zef' size='340' side='right'caption='[[5zef]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5zef]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZEF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NVA:NORVALINE'>NVA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zef OCA], [https://pdbe.org/5zef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zef RCSB], [https://www.ebi.ac.uk/pdbsum/5zef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zef ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ARGI_ENTH1 ARGI_ENTH1] Catalyzes the hydrolysis of L-arginine into urea and L-ornithine, which is a precursor for polyamine biosynthesis (PubMed:15053781, PubMed:29691540, PubMed:31199070). By depleting host L-arginine, a substrate for nitric oxide synthase (NOS), prevents the production of nitric oxide (NO) by host activated macrophages, and thus allows the parasite to evade host immune response (PubMed:15053781).<ref>PMID:15053781</ref> <ref>PMID:29691540</ref> <ref>PMID:31199070</ref> 
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== Publication Abstract from PubMed ==
Arginase, the binuclear metalloenzyme, is a potential target for therapeutic intervention in protozoan infections. Entamoeba histolytica infection causes amebiasis which is the second most common cause of protozoan-related human deaths after malaria. Here, we report the crystal structure of E. histolytica arginase (EhArg) in complex with two known inhibitors N(omega) -hydroxy-L-arginine (L-NOHA) and L-norvaline, and its product L-ornithine at 1.7 A, 2.0 A and 2.4 A respectively. Structural and comparative analysis of EhArg-inhibitor complexes with human arginase (HArg) revealed that despite of only 33% sequence identity, the structural determinants of inhibitor recognition and binding are highly conserved in arginases with variation in oligomerization motifs. Knowledge regarding the spatial organization of residues making molecular contacts with inhibitory compounds enabled in the identification of four novel non-amino acid inhibitors viz. irinotecan, argatroban, cortisone acetate and sorafenib. In vitro testing of the in silico identified inhibitors using purified enzyme proved that irinotecan, argatroban, cortisone acetate and sorafenib inhibit EhArg with IC50 value (mM) of 1.99, 2.40, 0.91 and 2.75 respectively as compared to the known inhibitors L-NOHA and L-norvaline with IC50 value (mM) of 1.57 and 17.9 respectively. Identification of structure-based non-amino acid inhibitory molecules against arginase will be constructive in design and discovery of novel chemical modulators for treating amebiasis by directed therapeutics. This article is protected by copyright. All rights reserved.

Structural insights into Entamoeba histolytica arginase and structure-based identification of novel non-amino acid based inhibitors as potential antiamoebic molecules.,Malik A, Dalal V, Ankri S, Tomar S FEBS J. 2019 Jun 14. doi: 10.1111/febs.14960. PMID:31199070<ref>PMID:31199070</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5zef" style="background-color:#fffaf0;"></div>

==See Also==
*[[Arginase 3D structures|Arginase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Entamoeba histolytica]]
[[Category: Large Structures]]
[[Category: Ankri S]]
[[Category: Dalal V]]
[[Category: Malik A]]
[[Category: Tomar S]]