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==Solution structure of G-quadruplex formed in vegfr-2 proximal promoter sequence==
<StructureSection load='5zev' size='340' side='right'caption='[[5zev]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5zev]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZEV FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zev OCA], [https://pdbe.org/5zev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zev RCSB], [https://www.ebi.ac.uk/pdbsum/5zev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zev ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Tumor angiogenesis is mainly regulated by vascular endothelial growth factor (VEGF), produced by cancer cells. It is active on the endothelium via VEGF receptor 2 (VEGFR-2). G-quadruplexes are DNA secondary structures formed by guanine-rich sequences, for example, within gene promoters where they may contribute to transcriptional activity. The proximal promoter of vegfr-2 contains a G-quadruplex, which has been suggested to interact with small molecules that inhibit VEGFR-2 expression and thereby tumor angiogenesis. However, its structure is not known. Here, we determined its NMR solution structure, which is composed of three stacked G-tetrads containing three syn guanines. The first guanine (G1) is positioned within the central G-tetrad. We also observed that a noncanonical, V-shaped loop spans three G-tetrad planes, including no bridging nucleotides. A long and diagonal loop, which includes six nucleotides, connects reversal double chains. With a melting temperature of 54.51 degrees C, the scaffold of this quadruplex is stabilized by one G-tetrad plane stacking with one nonstandard base pair, G3-C8, whose bases interact with each other through only one hydrogen bond. In summary, the NMR solution structure of the G-quadruplex in the proximal promoter region of the VEGFR-2 gene reported here has uncovered its key features as a potential anticancer drug target.

A putative G-quadruplex structure in the proximal promoter of vegfr-2 has implications for drug design to inhibit tumor angiogenesis.,Liu Y, Lan W, Wang C, Cao C J Biol Chem. 2018 Apr 17. pii: RA118.002666. doi: 10.1074/jbc.RA118.002666. PMID:29666187<ref>PMID:29666187</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
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</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Lan WX]]
[[Category: Liu Y]]