View source for 5zgm


==Crystal Structure of Parvalbumin SPVI, the Major Allergens in Mustelus griseus==
<StructureSection load='5zgm' size='340' side='right'caption='[[5zgm]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5zgm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mustelus_griseus Mustelus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZGM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.399&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zgm OCA], [https://pdbe.org/5zgm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zgm RCSB], [https://www.ebi.ac.uk/pdbsum/5zgm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zgm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PRVA_TRISE PRVA_TRISE] In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Fish play important roles in human nutrition and health, but also trigger allergic reactions in some population. Parvalbumin (PV) represents the major allergen of fish. While IgE cross-reactivity to PV in various bony fish species has been well characterized, little information is available about allergens in cartilaginous fish. In this study, two shark PV isoforms (named as SPV-I and SPV-II) from Mustelus griseus were purified. Their identities were further confirmed by mass spectroscopic analysis. IgE immunoblot analysis showed that sera from fish-allergic patients reacted to both SPV-I and SPV-II, but the majority of sera reacted more intensely to SPV-I than SPV-II. Thermal denaturation monitored by CD spectrum showed that both of the SPV allergens are highly thermostable. SPV-I maintained its IgE-binding capability after heat denaturation, while the IgE-binding capability of SPV-II was reduced. The results of crystal structure showed that SPV-I and SPV-II were similar in their overall tertiary structure, but their amino acid sequences shared lower similarities, indicating that the differences in the IgE-binding capabilities of SPV-I and SPV-II might be due to differential antigen epitopes in these two isoforms.

Purification, Characterization, and Crystal Structure of Parvalbumins, the Major Allergens in Mustelus griseus.,Yang RQ, Chen YL, Chen F, Wang H, Zhang Q, Liu GM, Jin T, Cao MJ J Agric Food Chem. 2018 Jul 17. doi: 10.1021/acs.jafc.8b01889. PMID:29969026<ref>PMID:29969026</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5zgm" style="background-color:#fffaf0;"></div>

==See Also==
*[[Parvalbumin|Parvalbumin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mustelus griseus]]
[[Category: Cao MJ]]
[[Category: Chen YL]]
[[Category: Jin TC]]
[[Category: Yang RQ]]