View source for 5zic


==Crystal structure of human GnT-V luminal domain in complex with acceptor sugar==
<StructureSection load='5zic' size='340' side='right'caption='[[5zic]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5zic]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZIC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=Z4Y:6-thio-alpha-D-mannopyranose'>Z4Y</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zic OCA], [https://pdbe.org/5zic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zic RCSB], [https://www.ebi.ac.uk/pdbsum/5zic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zic ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MGT5A_HUMAN MGT5A_HUMAN] Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
N-acetylglucosaminyltransferase-V (GnT-V) alters the structure of specific N-glycans by modifying alpha1-6-linked mannose with a beta1-6-linked N-acetylglucosamine branch. beta1-6 branch formation on cell surface receptors accelerates cancer metastasis, making GnT-V a promising target for drug development. However, the molecular basis of GnT-V's catalytic mechanism and substrate specificity are not fully understood. Here, we report crystal structures of human GnT-V luminal domain with a substrate analog. GnT-V luminal domain is composed of a GT-B fold and two accessary domains. Interestingly, two aromatic rings sandwich the alpha1-6 branch of the acceptor N-glycan and restrain the global conformation, partly explaining the fine branch specificity of GnT-V. In addition, interaction of the substrate N-glycoprotein with GnT-V likely contributes to protein-selective and site-specific glycan modification. In summary, the acceptor-GnT-V complex structure suggests a catalytic mechanism, explains the previously observed inhibition of GnT-V by branching enzyme GnT-III, and provides a basis for the rational design of drugs targeting N-glycan branching.

Structure and mechanism of cancer-associated N-acetylglucosaminyltransferase-V.,Nagae M, Kizuka Y, Mihara E, Kitago Y, Hanashima S, Ito Y, Takagi J, Taniguchi N, Yamaguchi Y Nat Commun. 2018 Aug 23;9(1):3380. doi: 10.1038/s41467-018-05931-w. PMID:30140003<ref>PMID:30140003</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5zic" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Nagae M]]
[[Category: Yamaguchi Y]]