View source for 5zuu


==Crystal structure of AtCPSF30 YTH domain in complex with 10mer m6A-modified RNA==
<StructureSection load='5zuu' size='340' side='right'caption='[[5zuu]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5zuu]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZUU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6MD:N-METHYLADENOSINE'>6MD</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zuu OCA], [https://pdbe.org/5zuu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zuu RCSB], [https://www.ebi.ac.uk/pdbsum/5zuu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zuu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CPSF_ARATH CPSF_ARATH] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation. May interact with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition (By similarity). Mediates poly(A) site selection (PubMed:23136375). Binds RNA in a calcium-dependent manner (PubMed:16500995, PubMed:17576667, PubMed:20214900). Exhibits endonuclease activity with an ability to nick and degrade linear as well as circular single-stranded RNA that leaves RNA 3' ends with hydroxyl groups, thus mediating processing of the pre-mRNA as a prelude to the polyadenylation (PubMed:17576667). Involved in the post-transcriptional control, probably via poly(A) addition, of the responses of plants to stress, especially genes mediating tolerance to oxidative stress (PubMed:18545667). Plays a role in the regulation of salicylic acid (SA) production via the control of messenger RNA 3' end processing, thus being a key component of programmed cell death and plant immune responses required for resistance to virulent Pseudomonas syringae pv tomato DC3000 (Pst) (PubMed:24706550).[UniProtKB:O95639]<ref>PMID:16500995</ref> <ref>PMID:17576667</ref> <ref>PMID:18545667</ref> <ref>PMID:20214900</ref> <ref>PMID:23136375</ref> <ref>PMID:24706550</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Li S]]
[[Category: Nie H]]
[[Category: Patel DJ]]
[[Category: Wu B]]