View source for 5zxu


==Crystal structure of CurA in complex with NADPH from Vibrio vulnificus==
<StructureSection load='5zxu' size='340' side='right'caption='[[5zxu]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5zxu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_vulnificus_MO6-24/O Vibrio vulnificus MO6-24/O]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZXU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zxu OCA], [https://pdbe.org/5zxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zxu RCSB], [https://www.ebi.ac.uk/pdbsum/5zxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zxu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A4V8GZL4_VIBVM A0A4V8GZL4_VIBVM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Curcumin is a yellow-colored ingredient in dietary spice turmeric ( Curcuma longa Linn). This nontoxic polyphenol has antitumor, anti-inflammatory, apoptotic, and antioxidant activities. The ingested curcumin is reduced to multihydrated forms with more potent therapeutic potentials by the curcumin reductase (CurA) from commensal Escherichia coli. In this study, we demonstrated that Vibrio vulnificus CurA ( VvCurA) with 87% sequence similarity to the E. coli CurA exhibits the curcumin-reducing activity through spectrophotometric detection of NADPH oxidation and high performance liquid chromatographic analysis of curcumin consumption and product generation. Afterward, we determined the crystal structures of VvCurA and the VvCurA/NADPH complex, and made the in silico model of the VvCurA/NADPH/curcumin ternary complex through induced fit docking. Based on structural information, active site residues that play critical roles in catalysis have been identified and characterized by mutational and kinetic studies, leading us to propose the reaction mechanism of CurA.

Structural and Biochemical Characterization of the Curcumin-Reducing Activity of CurA from Vibrio vulnificus.,Park SB, Bae DW, Clavio NAB, Zhao L, Jeong CS, Choi BM, Macalino SJY, Cha HJ, Park JB, Lee JH, Nam SJ, Choi S, Kim MK, Cha SS J Agric Food Chem. 2018 Oct 10;66(40):10608-10616. doi: 10.1021/acs.jafc.8b03647., Epub 2018 Oct 2. PMID:30251539<ref>PMID:30251539</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5zxu" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio vulnificus MO6-24/O]]
[[Category: Bae D-W]]
[[Category: Cha S-S]]
[[Category: Kim M-K]]