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==Novel Regulators CheP and CheQ Specifically Control Chemotaxis Core Gene cheVAW Transcription in Bacterial Pathogen Campylobacter jejuni==
<StructureSection load='6a51' size='340' side='right'caption='[[6a51]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6a51]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni Campylobacter jejuni subsp. jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A51 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a51 OCA], [https://pdbe.org/6a51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a51 RCSB], [https://www.ebi.ac.uk/pdbsum/6a51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a51 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q0PBQ6_CAMJE Q0PBQ6_CAMJE] 
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== Publication Abstract from PubMed ==
Campylobacter jejuni is the leading cause of foodborne gastrointestinal illness worldwide, and chemotaxis plays an important role in its host colonization and pathogenesis. Although many studies on chemotaxis have focused on the physical organization and signaling mechanism of the system's protein complex, much less is known about the transcriptional regulation of its components. Here, we describe two novel regulators, CJJ81176_0275 and CJJ81176_0276 (designated as CheP and CheQ), which specifically activate the transcription of the chemotaxis core genes cheV, cheA and cheW in C. jejuni and they are also essential for chemotactic responses. CheP has a single HD-related output domain (HDOD) domain and can promote CheQ binding to the cheVAW operon promoter through a protein-protein interaction. Mutagenesis analyses identified key residues critical for CheP function and/or interaction with CheQ. Further structural characterization of CheQ revealed a novel fold with strong positive surface charges that allow for its DNA binding. These findings reveal the gene regulatory mechanism of the chemotaxis system in an important bacterial pathogen and provide potential anti-virulence targets for campylobacteriosis treatment. In addition, ChePQ is an example of how proteins with the widespread but functionally obscure HDOD can coordinate with a signal output DNA-binding protein/domain to regulate the expression of important signaling pathways.

The novel regulators CheP and CheQ control the core chemotaxis operon cheVAW in Campylobacter jejuni.,Cha G, Chen Z, Mo R, Lu G, Gao B Mol Microbiol. 2019 Jan;111(1):145-158. doi: 10.1111/mmi.14144. Epub 2018 Nov 22. PMID:30338872<ref>PMID:30338872</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6a51" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Campylobacter jejuni subsp. jejuni]]
[[Category: Large Structures]]
[[Category: Cha G]]
[[Category: Chen Z]]
[[Category: Gao B]]
[[Category: Lu G]]
[[Category: Mo R]]