View source for 6ax2


==Solution structure of Magi3 a specific insect toxin from the spider Macrothele gigas==
<StructureSection load='6ax2' size='340' side='right'caption='[[6ax2]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ax2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macrothele_gigas Macrothele gigas]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AX2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AX2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 12 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ax2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ax2 OCA], [https://pdbe.org/6ax2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ax2 RCSB], [https://www.ebi.ac.uk/pdbsum/6ax2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ax2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TX22A_MACGS TX22A_MACGS] Competes for binding at site 3 of the insect voltage-gated sodium channel (Nav) (PubMed:12860384). Insecticidal neurotoxin (PubMed:12860384, PubMed:29247580). Causes temporary paralysis to lepidopteran larvae (10.3 nmol/g) or to crickets (doses from 0.93 to 119 ug/g) (PubMed:12860384, PubMed:29247580). Is not toxic to mice when injected intracranially (high doses) (PubMed:12860384, PubMed:29247580).<ref>PMID:12860384</ref> <ref>PMID:29247580</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly selective toxins for insect preys. In this respect, spiders as a highly diversified group of almost exclusive insect predators appear to possess infinite potential for the discovery of novel insect-selective toxins. In 2003, a group of toxins was isolated from the spider Macrothele gigas and the amino acid sequence was reported. We obtained, by molecular biology techniques in a heterologous system, one of these toxins. Purification process was optimized by chromatographic methods to determine the three-dimensional structure by nuclear magnetic resonance in solution, and, finally, their biological activity was tested. rMagi3 resulted to be a specific insect toxin with no effect on mice.

Successful refolding and NMR structure of rMagi3: a disulfide-rich insecticidal spider toxin.,Titaux-Delgado G, Carrillo E, Mendoza A, Mayorga-Flores M, Escobedo-Gonzalez FC, Cano-Sanchez P, Lopez-Vera E, Corzo G, Del Rio-Portilla F Protein Sci. 2017 Dec 16. doi: 10.1002/pro.3363. PMID:29247580<ref>PMID:29247580</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ax2" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Macrothele gigas]]
[[Category: Escobedo-Gonzalez FC]]
[[Category: Titaux-Delgado GA]]
[[Category: Del Rio-Portilla F]]