View source for 6b55


==Crystal structure of the Plant Defensin NaD1 complexed with phosphatidic acid==
<StructureSection load='6b55' size='340' side='right'caption='[[6b55]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6b55]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_alata Nicotiana alata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B55 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PA8:1,2-DIOCTANOYL-SN-GLYCERO-3-PHOSPHATE'>PA8</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b55 OCA], [https://pdbe.org/6b55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b55 RCSB], [https://www.ebi.ac.uk/pdbsum/6b55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b55 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DEF_NICAL DEF_NICAL] Plant defense peptide with antifungal activity against F.oxysporum and B.cinerea. Retards the growth of the Lepidopteran insect pests H.armigera and H.punctigera.
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== Publication Abstract from PubMed ==
Defensins are cationic antimicrobial peptides expressed throughout the plant and animal kingdoms as a first line of defense against pathogens. Membrane targeting and disruption is a crucial function of many defensins, however the precise mechanism remains unclear. Certain plant defensins form dimers that specifically bind the membrane phospholipids phosphatidic acid (PA) and phosphatidylinositol 4,5-bisphosphate, thereby triggering the assembly of defensin-lipid oligomers that permeabilize cell membranes. To understand this permeabilization mechanism, here we determine the crystal structure of the plant defensin NaD1 bound to PA. The structure reveals a 20-mer that adopts a concave sheet- or carpet-like topology where NaD1 dimers form one face and PA acyl chains form the other face of the sheet. Furthermore, we show that Arg39 is critical for PA binding, oligomerization and fungal cell killing. These findings identify a putative defensin-phospholipid membrane attack configuration that supports a longstanding proposed carpet mode of membrane disruption.

X-ray structure of a carpet-like antimicrobial defensin-phospholipid membrane disruption complex.,Jarva M, Lay FT, Phan TK, Humble C, Poon IKH, Bleackley MR, Anderson MA, Hulett MD, Kvansakul M Nat Commun. 2018 May 17;9(1):1962. doi: 10.1038/s41467-018-04434-y. PMID:29773800<ref>PMID:29773800</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6b55" style="background-color:#fffaf0;"></div>

==See Also==
*[[Defensin 3D structures|Defensin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Nicotiana alata]]
[[Category: Hulett M]]
[[Category: Humble C]]
[[Category: Jarva M]]
[[Category: Kvansakul M]]
[[Category: Lay FT]]
[[Category: Phan K]]