View source for 6baf


==Structure of the chromophore binding domain of Stigmatella aurantiaca phytochrome P1, wild-type==
<StructureSection load='6baf' size='340' side='right'caption='[[6baf]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6baf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stigmatella_aurantiaca_DW4/3-1 Stigmatella aurantiaca DW4/3-1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4rpw 4rpw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BAF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BAF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLR:3-[5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-2-[[5-[(Z)-(3-ethenyl-4-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1H-pyrrol-2-yl]methyl]-4-methyl-1H-pyrrol-3-yl]propanoic+acid'>BLR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6baf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6baf OCA], [https://pdbe.org/6baf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6baf RCSB], [https://www.ebi.ac.uk/pdbsum/6baf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6baf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q097N3_STIAD Q097N3_STIAD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phytochromes are red-light photoreceptors that were first characterized in plants, with homologs in photosynthetic and non-photosynthetic bacteria known as bacteriophytochromes (BphPs). Upon absorption of light, BphPs interconvert between two states denoted Pr and Pfr with distinct absorption spectra in the red and far-red. They have recently been engineered as enzymatic photoswitches for fluorescent-marker applications in non-invasive tissue imaging of mammals. This article presents cryo- and room-temperature crystal structures of the unusual phytochrome from the non-photosynthetic myxo-bacterium Stigmatella aurantiaca (SaBphP1) and reveals its role in the fruiting-body formation of this photomorphogenic bacterium. SaBphP1 lacks a conserved histidine (His) in the chromophore-binding domain that stabilizes the Pr state in the classical BphPs. Instead it contains a threonine (Thr), a feature that is restricted to several myxobacterial phytochromes and is not evolutionarily understood. SaBphP1 structures of the chromophore binding domain (CBD) and the complete photosensory core module (PCM) in wild-type and Thr-to-His mutant forms reveal details of the molecular mechanism of the Pr/Pfr transition associated with the physiological response of this myxobacterium to red light. Specifically, key structural differences in the CBD and PCM between the wild-type and the Thr-to-His mutant involve essential chromophore contacts with proximal amino acids, and point to how the photosignal is transduced through the rest of the protein, impacting the essential enzymatic activity in the photomorphogenic response of this myxobacterium.

Structural basis for light control of cell development revealed by crystal structures of a myxobacterial phytochrome.,Woitowich NC, Halavaty AS, Waltz P, Kupitz C, Valera J, Tracy G, Gallagher KD, Claesson E, Nakane T, Pandey S, Nelson G, Tanaka R, Nango E, Mizohata E, Owada S, Tono K, Joti Y, Nugent AC, Patel H, Mapara A, Hopkins J, Duong P, Bizhga D, Kovaleva SE, St Peter R, Hernandez CN, Ozarowski WB, Roy-Chowdhuri S, Yang JH, Edlund P, Takala H, Ihalainen J, Brayshaw J, Norwood T, Poudyal I, Fromme P, Spence JCH, Moffat K, Westenhoff S, Schmidt M, Stojkovic EA IUCrJ. 2018 Aug 29;5(Pt 5):619-634. doi: 10.1107/S2052252518010631. eCollection, 2018 Sep 1. PMID:30224965<ref>PMID:30224965</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6baf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Stigmatella aurantiaca DW4/3-1]]
[[Category: Schmidt M]]
[[Category: Stojkovic E]]