View source for 6bbt


==Structure of the major pilin protein (T-13) from Streptococcus pyogenes serotype GAS131465==
<StructureSection load='6bbt' size='340' side='right'caption='[[6bbt]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6bbt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BBT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BBT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bbt OCA], [https://pdbe.org/6bbt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bbt RCSB], [https://www.ebi.ac.uk/pdbsum/6bbt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bbt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A2T8Y3_STRPY A2T8Y3_STRPY] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Group A Streptococcus (GAS, Streptococcus pyogenes) is an important human pathogen associated with significant global morbidity and mortality for which there is no safe and efficacious vaccine. The T-antigen, a protein that polymerises to form the backbone of the GAS pilus structure, is a potential vaccine candidate. Previous surveys of the tee gene, which encodes the T-antigen, have identified 21 different tee-types and subtypes such that any T-antigen-based vaccine must be multivalent and carefully designed to provide broad strain coverage. In this study, the crystal structures of three two-domain T-antigens (T3.2, T13 and T18.1) were determined and found to have remarkable structural similarity to the previously reported T1, despite moderate overall sequence similarity. This has enabled reliable modelling of all major two-domain T-antigens to reveal that T-antigen sequence variation is distributed along the full length of the protein, and shields a highly conserved core. Immunoassays performed with sera from immunized animals and commercial T-typing sera identified a significant cross-reactive antibody response between T18.1, T18.2, T3.2 and T13. The existence of shared epitopes between T-antigens, combined with the remarkably conserved structure and high surface sequence divergence, has important implications for the design of multivalent T-antigen-based vaccines.

Group A Streptococcus T-antigens have a highly conserved structure concealed under a heterogeneous surface that has implications for vaccine design.,Young PG, Raynes JM, Loh JM, Proft T, Baker EN, Moreland NJ Infect Immun. 2019 Apr 1. pii: IAI.00205-19. doi: 10.1128/IAI.00205-19. PMID:30936156<ref>PMID:30936156</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6bbt" style="background-color:#fffaf0;"></div>

==See Also==
*[[Pilin 3D structures|Pilin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus pyogenes]]
[[Category: Baker EN]]
[[Category: Moreland NJ]]
[[Category: Young PG]]