View source for 6bul


==Crystal structure of Staphylococcus aureus ketol-acid reductoisomerase with hydroxyoxamate inhibitor 2==
<StructureSection load='6bul' size='340' side='right'caption='[[6bul]], [[Resolution|resolution]] 1.88&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6bul]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_RF122 Staphylococcus aureus RF122]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BUL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.88&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E9G:{hydroxy[(1S)-1-phenylethyl]amino}(oxo)acetic+acid'>E9G</scene>, <scene name='pdbligand=E9J:oxo{[(1S)-1-phenylethyl]amino}acetic+acid'>E9J</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bul OCA], [https://pdbe.org/6bul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bul RCSB], [https://www.ebi.ac.uk/pdbsum/6bul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bul ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ILVC_STAAB ILVC_STAAB] Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.

==See Also==
*[[Ketol-acid reductoisomerase 3D structures|Ketol-acid reductoisomerase 3D structures]]
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</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus RF122]]
[[Category: Guddat LW]]
[[Category: Kandale A]]
[[Category: McFeary RP]]
[[Category: Patel KM]]
[[Category: Schembri MA]]
[[Category: Schenk G]]
[[Category: You L]]
[[Category: Zheng S]]