View source for 6bvq


==Crystal structure of 3-hydroxyanthranilate-3,4-dioxygenase N27A from Cupriavidus metallidurans in complex with 4-Cl-3-HAA==
<StructureSection load='6bvq' size='340' side='right'caption='[[6bvq]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6bvq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_metallidurans Cupriavidus metallidurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BVQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.084&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4AA:4-CHLORO-3-HYDROXYANTHRANILIC+ACID'>4AA</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bvq OCA], [https://pdbe.org/6bvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bvq RCSB], [https://www.ebi.ac.uk/pdbsum/6bvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bvq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/3HAO_CUPMC 3HAO_CUPMC] Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.[HAMAP-Rule:MF_00825]<ref>PMID:15909977</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
3-Hydroxyanthranilate 3,4-dioxygenase (HAO) is an iron-dependent protein that activates O2 and inserts both O atoms into 3-hydroxyanthranilate (3-HAA). An intriguing question is how HAO can rapidly bind O2, even though local O2 concentrations and diffusion rates are relatively low. Here, a close inspection of the HAO structures revealed that substrate- and inhibitor-bound structures exhibit a closed conformation with three hydrophobic loop regions moving toward the catalytic iron center, whereas the ligand-free structure is open. We hypothesized that these loop movements enhance O2 binding to the binary complex of HAO and to 3-HAA. We found that the carboxyl end of 3-HAA triggers the changes in two loop regions and that the third loop movement appears to be driven by an H-bond interaction between Asn-27 and Ile-142. Mutational analyses revealed that N27A, I142A, and I142P variants cannot form a closed conformation, and steady-state kinetic assays indicated that these variants have a substantially higher Km for O2 than wild-type HAO. This observation suggested enhanced hydrophobicity at the iron center resulting from the concerted loop movements after the binding of the primary substrate, which is hydrophilic. Given that O2 is nonpolar, the increased hydrophobicity at the Fe center of the complex appears to be essential for rapid O2 binding and activation, explaining the reason for the 3-HAA-induced loop movements. As substrate binding-induced open-to-closed conformational changes are common, the results reported here may help further our understanding of how oxygen is enriched in the nonheme Fe-dependent dioxygenases.

Adapting to oxygen: 3-Hydroxyanthrinilate 3,4-dioxygenase employs loop dynamics to accommodate two substrates with disparate polarities.,Yang Y, Liu F, Liu A J Biol Chem. 2018 May 21. pii: RA118.002698. doi: 10.1074/jbc.RA118.002698. PMID:29784877<ref>PMID:29784877</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6bvq" style="background-color:#fffaf0;"></div>

==See Also==
*[[Dioxygenase 3D structures|Dioxygenase 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Cupriavidus metallidurans]]
[[Category: Large Structures]]
[[Category: Liu A]]
[[Category: Liu F]]
[[Category: Yang Y]]