View source for 6ccr


==Selenomethionyl derivative of a GID4 fragment==
<StructureSection load='6ccr' size='340' side='right'caption='[[6ccr]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ccr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CCR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ccr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ccr OCA], [https://pdbe.org/6ccr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ccr RCSB], [https://www.ebi.ac.uk/pdbsum/6ccr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ccr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GID4_HUMAN GID4_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The N-end rule pathway senses the N-terminal destabilizing residues of degradation substrates for the ubiquitin-proteasome system, whose integrity shields against various human syndromes including cancer and cardiovascular diseases. GID4, a subunit of the ubiquitin ligase GID complex, has been recently identified as the N-recognin of the new branch of the N-end rule pathway responsible for recognizing substrates bearing N-terminal proline residues (Pro/N-degrons). However, the molecular mechanism of GID4-mediated Pro/N-degron recognition remains largely unexplored. Here, we report the first crystal structures of human GID4 alone and in complex with various Pro/N-degrons. Our complex crystal structures, together with biophysical analyses, delineate the GID4-mediated Pro/N-degron recognition mechanism and substrate selection criteria for the Pro/N-end rule pathway. These mechanistic data on the Pro/N-recognin activity of GID4 will serve as a foundation to facilitate the identification of authentic physiological substrates as well as the development of inhibitors of therapeutic values for the Pro/N-end rule pathway.

Molecular basis of GID4-mediated recognition of degrons for the Pro/N-end rule pathway.,Dong C, Zhang H, Li L, Tempel W, Loppnau P, Min J Nat Chem Biol. 2018 May;14(5):466-473. doi: 10.1038/s41589-018-0036-1. Epub 2018 , Apr 9. PMID:29632410<ref>PMID:29632410</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ccr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Arrowsmith CH]]
[[Category: Bountra C]]
[[Category: Dong C]]
[[Category: Edwards AM]]
[[Category: Min J]]
[[Category: Tempel W]]