View source for 6d8g


==D341A D367A calcium binding mutant of Bacteroides uniformis beta-glucuronidase 2==
<StructureSection load='6d8g' size='340' side='right'caption='[[6d8g]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6d8g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_uniformis_str._3978_T3_ii Bacteroides uniformis str. 3978 T3 ii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D8G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D8G FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d8g OCA], [https://pdbe.org/6d8g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d8g RCSB], [https://www.ebi.ac.uk/pdbsum/6d8g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d8g ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A078SUX9_BACUN A0A078SUX9_BACUN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The carbohydrates and glycoconjugates that enter the human gastrointestinal tract (GI) are remarkably complex, and most of the genes encoding the enzymes required for processing these molecules are not present in the human genome. The glycoside hydrolases encoded by the human gut microbiome therefore play an integral role in processing these diverse exogenous and endogenous glycoconjugates. Here, we analyzed three structurally and functionally distinct beta-glucuronidase (GUS) glycoside hydrolases from a single commensal microbe of the human gut, Bacteroides uniformis. Using nine crystal structures and biochemical and biophysical data, we show that although these three proteins share similar overall folds, they exhibit different structural features that create three structurally and functionally unique active sites. Notably, we found that quaternary structure plays an important role in creating distinct active-site features that are difficult to predict via structural modeling methods alone. The enzymes displayed differential processing capabilities toward several glucuronic acid-containing polysaccharides. They also hydrolyzed SN-38-glucuronide, a metabolite of the anticancer drug irinotecan, suggesting that these gut-microbial enzymes could reactivate this drug in the GI. We further demonstrate that GUS-specific and non-selective inhibitors exhibit varying potencies toward each enzyme. Together, these results highlight the diversity of GUS enzymes within a single Bacteroides gut commensal and advance our understanding of how structural properties affect the specific roles of microbial enzymes in processing and catabolizing drug-derived glucuronides and glycan substrates.

Three structurally and functionally distinct beta-glucuronidases from the human gut microbe Bacteroides uniformis.,Pellock SJ, Walton WG, Biernat KA, Torres-Rivera D, Creekmore BC, Xu Y, Liu J, Tripathy A, Stewart LJ, Redinbo MR J Biol Chem. 2018 Oct 9. pii: RA118.005414. doi: 10.1074/jbc.RA118.005414. PMID:30301767<ref>PMID:30301767</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6d8g" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glucuronisidase 3D structures|Glucuronisidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacteroides uniformis str. 3978 T3 ii]]
[[Category: Large Structures]]
[[Category: Pellock SJ]]
[[Category: Redinbo MR]]
[[Category: Walton WG]]