View source for 6dam


==Crystal structure of lanthanide-dependent methanol dehydrogenase XoxF from Methylomicrobium buryatense 5G==
<StructureSection load='6dam' size='340' side='right'caption='[[6dam]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6dam]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylotuvimicrobium_buryatense_5G Methylotuvimicrobium buryatense 5G]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DAM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dam OCA], [https://pdbe.org/6dam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dam RCSB], [https://www.ebi.ac.uk/pdbsum/6dam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dam ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A3F2YLY8_9GAMM A0A3F2YLY8_9GAMM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In methylotrophic bacteria, which use one-carbon (C1) compounds as a carbon source, methanol is oxidized by pyrroloquinoline quinone (PQQ)-dependent methanol dehydrogenase (MDH) enzymes. Methylotrophic genomes generally encode two distinct MDHs, MxaF and XoxF. MxaF is a well-studied, calcium-dependent heterotetrameric enzyme whereas XoxF is a lanthanide-dependent homodimer. Recent studies suggest that XoxFs are likely the functional MDHs in many environments. In methanotrophs, methylotrophs that utilize methane, interactions between particulate methane monooxygenase (pMMO) and MxaF have been detected. To investigate the possibility of interactions between pMMO and XoxF, XoxF was isolated from the methanotroph Methylomicrobium buryatense 5GB1C (5G-XoxF). Purified 5G-XoxF exhibits a specific activity of 0.16 mumol DCPIP reduced min(-1) mg(-1). The 1.85 A resolution crystal structure reveals a La(III) ion in the active site, in contrast to the calcium ion in MxaF. The overall fold is similar to other MDH structures, but 5G-XoxF is a monomer in solution. An interaction between 5G-XoxF and its cognate pMMO was detected by biolayer interferometry, with a KD value of 50 +/- 17 muM. These results suggest an alternative model of MDH-pMMO association, in which a XoxF monomer may bind to pMMO, and underscore the potential importance of lanthanide-dependent MDHs in biological methane oxidation.

Structure and function of the lanthanide-dependent methanol dehydrogenase XoxF from the methanotroph Methylomicrobium buryatense 5GB1C.,Deng YW, Ro SY, Rosenzweig AC J Biol Inorg Chem. 2018 Aug 21. pii: 10.1007/s00775-018-1604-2. doi:, 10.1007/s00775-018-1604-2. PMID:30132076<ref>PMID:30132076</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6dam" style="background-color:#fffaf0;"></div>

==See Also==
*[[Methanol dehydrogenase|Methanol dehydrogenase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methylotuvimicrobium buryatense 5G]]
[[Category: Deng Y]]
[[Category: Ro SY]]
[[Category: Rosenzweig AC]]