View source for 6dfe


==The structure of a ternary complex of E. coli WaaC==
<StructureSection load='6dfe' size='340' side='right'caption='[[6dfe]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6dfe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DFE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.31&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GA7:[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl+(2R)-1-{(2S,3S,4R,5S,6R)-6-[(1S)-1,2-dihydroxyethyl]-3,4,5-trihydroxytetrahydro-2H-pyran-2-yl}propan-2-yl+hydrogen+(R)-phosphate+(non-preferred+name)'>GA7</scene>, <scene name='pdbligand=GN1:2-(ACETYLAMINO)-2-DEOXY-1-O-PHOSPHONO-ALPHA-D-GLUCOPYRANOSE'>GN1</scene>, <scene name='pdbligand=GN4:2-acetamido-2-deoxy-4-O-phosphono-beta-D-glucopyranose'>GN4</scene>, <scene name='pdbligand=KDO:3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID'>KDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dfe OCA], [https://pdbe.org/6dfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dfe RCSB], [https://www.ebi.ac.uk/pdbsum/6dfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dfe ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A152KUZ3_ECOLX A0A152KUZ3_ECOLX] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Heptosyltransferase I (WaaC) is a highly conserved glycosyltransferase found in Gram-negative bacteria that transfers a heptose residue onto the endotoxin inner core structure (ReLPS) of the outer membrane. Knockouts of WaaC have decreased virulence and increased susceptibility to antibiotics, making WaaC a potential drug target. While previous studies have elucidated the structure of the holoenzyme and a donor analog complex, no information on the binding mode of the acceptor has been available so far. By soaking of a chemically modified functional acceptor, along with a stable donor analog, the crystal structure of a pseudo-ternary complex of WaaC was obtained at 2.3-A resolution. The acceptor is bound in an unusual horseshoe conformation stabilized by interaction of the anionic carboxylate and phosphate groups at its center and tips with highly conserved Lys and Arg residues. This binding is accompanied by both inter- and intra-domain movements within the protein.

Insights into Heptosyltransferase I Catalysis and Inhibition through the Structure of Its Ternary Complex.,Blaukopf M, Worrall L, Kosma P, Strynadka NCJ, Withers SG Structure. 2018 Jul 23. pii: S0969-2126(18)30247-8. doi:, 10.1016/j.str.2018.07.001. PMID:30122450<ref>PMID:30122450</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6dfe" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Blaukopf M]]
[[Category: Strynadka NCJ]]
[[Category: Withers SG]]
[[Category: Worrall LJ]]