View source for 6dgu


==PER-2 class A extended-spectrum beta-lactamase crystal structure at 2.69 Angstrom resolution==
<StructureSection load='6dgu' size='340' side='right'caption='[[6dgu]], [[Resolution|resolution]] 2.69&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6dgu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DGU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.691&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dgu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgu OCA], [https://pdbe.org/6dgu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dgu RCSB], [https://www.ebi.ac.uk/pdbsum/6dgu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A2RP81_CITFR A2RP81_CITFR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The diazabicyclooctane (DBO) avibactam (AVI) reversibly inactivates most serine-beta-lactamases. Previous investigations showed that inhibition constants of AVI toward class A PER-2 are reminiscent of values observed for class C and D beta-lactamases (i.e., k 2/K of approximately 10(3) M(-1) s(-1)) but lower than other class A beta-lactamases (i.e., k 2/K = 10(4) to 10(5) M(-1) s(-1)). Herein, biochemical and structural studies were conducted with PER-2 and AVI to explore these differences. Furthermore, biochemical studies on Arg220 and Thr237 variants with AVI were conducted to gain deeper insight into the mechanism of PER-2 inactivation. The main biochemical and structural observations revealed the following: (i) both amino-acid substitutions in Arg220 and the rich hydrophobic content in the active site hinder the binding of catalytic waters and acylation, impairing AVI inhibition; (ii) movement of Ser130 upon binding of AVI favors the formation of a hydrogen bond with the sulfate group of AVI; and (iii) the Thr237Ala substitution alters the AVI inhibition constants. The acylation constant (k 2/K) of PER-2 by AVI is primarily influenced by stabilizing hydrogen bonds involving AVI and important residues such as Thr237 and Arg220. (Variants in Arg220 demonstrate a dramatic reduction in k 2/K) We also observed that displacement of Ser130 side chain impairs AVI acylation, an observation not made in other extended-spectrum beta-lactamases (ESBLs). Comparatively, relebactam combined with a beta-lactam is more potent against Escherichia coli producing PER-2 variants than beta-lactam-AVI combinations. Our findings provide a rationale for evaluating the utility of the currently available DBO inhibitors against unique ESBLs like PER-2 and anticipate the effectiveness of these inhibitors toward variants that may eventually be selected upon AVI usage.

Structural Insights into the Inhibition of the Extended-Spectrum beta-Lactamase PER-2 by Avibactam.,Ruggiero M, Papp-Wallace KM, Brunetti F, Barnes MD, Bonomo RA, Gutkind G, Klinke S, Power P Antimicrob Agents Chemother. 2019 Aug 23;63(9). pii: AAC.00487-19. doi:, 10.1128/AAC.00487-19. Print 2019 Sep. PMID:31235626<ref>PMID:31235626</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6dgu" style="background-color:#fffaf0;"></div>

==See Also==
*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Citrobacter freundii]]
[[Category: Large Structures]]
[[Category: Bonomo R]]
[[Category: Gutkind G]]
[[Category: Klinke S]]
[[Category: Power P]]
[[Category: Ruggiero M]]