View source for 6dwo


==Crystal structure of alpha-1-2-mannosidase from Enterococcus faecalis V583==
<StructureSection load='6dwo' size='340' side='right'caption='[[6dwo]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6dwo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis_V583 Enterococcus faecalis V583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DWO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dwo OCA], [https://pdbe.org/6dwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dwo RCSB], [https://www.ebi.ac.uk/pdbsum/6dwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dwo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q832K9_ENTFA Q832K9_ENTFA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
While multiple alpha 1-2-mannosidases are necessary for glycoprotein N-glycan maturation in vertebrates, a single bacterial alpha1-2-mannosidase can be sufficient to cleave all alpha1-2-linked mannose residues in host glycoprotein N-glycans. We report here the characterization and crystal structure of a new alpha1-2-mannosidase (EfMan-I) from Enterococcus faecalis, a Gram-positive opportunistic human pathogen. EfMan-I catalyzes the cleavage of alpha1-2-mannose from not only oligomannoses but also high-mannose-type N-glycans on glycoproteins. Its 2.15 A resolution crystal structure reveals a two-domain enzyme fold similar to other CAZy GH92 mannosidases. An unexpected potassium ion was observed bridging two domains near the active site. These findings support EfMan-I as an effective catalyst for in vitro N-glycan modification of glycoproteins with high-mannose-type N-glycans.

Enterococcus faecalis alpha1-2-mannosidase (EfMan-I): an efficient catalyst for glycoprotein N-glycan modification.,Li Y, Li R, Yu H, Sheng X, Wang J, Fisher AJ, Chen X FEBS Lett. 2019 Sep 25. doi: 10.1002/1873-3468.13618. PMID:31552675<ref>PMID:31552675</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6dwo" style="background-color:#fffaf0;"></div>

==See Also==
*[[Mannosidase 3D structures|Mannosidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Enterococcus faecalis V583]]
[[Category: Large Structures]]
[[Category: Fisher AJ]]
[[Category: Li Y]]