View source for 6dyx


==Structure of VHH R419 isolated from a pre-immune phage display library==
<StructureSection load='6dyx' size='340' side='right'caption='[[6dyx]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6dyx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DYX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dyx OCA], [https://pdbe.org/6dyx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dyx RCSB], [https://www.ebi.ac.uk/pdbsum/6dyx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dyx ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
OBJECTIVE: To determine the X-ray structure and biophysical properties of a Camelid VHH isolated from a naive phage display library. RESULTS: Single domain antibodies (VHH) derived from the unique immune system of the Camelidae family have gained traction as useful tools for biotechnology as well as a source of potentially novel therapeutics. Here we report the structure and biophysical characterization of a VHH originally isolated from a naive camelid phage display library. VHH R419 has a melting temperate of 66 degrees C and was found to be a monomer in solution. The protein crystallized in space group P6522 and the structure was solved by molecular replacement to a resolution of 1.5 A. The structure revealed a flat paratope with CDR loops that could be classified into existing canonical loop structures. A combination of high expression yield, stability and rapid crystallization might make R419 into a candidate scaffold for CDR grafting and homology modeling.

Structure of a VHH isolated from a naive phage display library.,White B, Huh I, Brooks CL BMC Res Notes. 2019 Mar 19;12(1):154. doi: 10.1186/s13104-019-4197-0. PMID:30890176<ref>PMID:30890176</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6dyx" style="background-color:#fffaf0;"></div>

==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vicugna pacos]]
[[Category: Brooks CL]]