View source for 6dz9


==Solution structure of Rbfox2 RRM mimetic peptide CPfox2==
<StructureSection load='6dz9' size='340' side='right'caption='[[6dz9]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6dz9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fischeri Aspergillus fischeri]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DZ9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dz9 OCA], [https://pdbe.org/6dz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dz9 RCSB], [https://www.ebi.ac.uk/pdbsum/6dz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dz9 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The RNA recognition motif (RRM), which is the most abundant RNA-binding motif in eukaryotes, is a well-structured domain of about 90 amino acids, yet the beta2beta3 hairpin, corresponding to strands 2 and 3 of the beta-sheet, and the intervening loop make essential interactions with RNA in many RRM complexes. A series of small cyclic peptide mimics of the beta2beta3 hairpin of Rbfox2 protein that recognize the terminal loop of precursor miR-20b have been designed to investigate whether the full RNA-binding protein can be mimicked with a minimal structurally preorganized peptide. Within a small library of seven cyclic peptides, a peptide with low-micromolar affinity for the miR-20b precursor was found. NMR spectroscopy titration data suggest that this peptide specifically targets the apical loop of pre-miR-20b. This work shows that it is possible to mimic RNA-binding proteins with designed stable peptides, which provide a starting point for designing or evolving small peptide mimetics of RRM proteins.

A Small Cyclic beta-Hairpin Peptide Mimics the Rbfox2 RNA Recognition Motif and Binds to the Precursor miRNA 20b.,Sun YT, Shortridge MD, Varani G Chembiochem. 2019 Apr 1;20(7):931-939. doi: 10.1002/cbic.201800645. Epub 2019 Feb, 15. PMID:30537200<ref>PMID:30537200</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6dz9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Aspergillus fischeri]]
[[Category: Large Structures]]
[[Category: Shortridge MD]]
[[Category: Sun Y-T]]
[[Category: Varani G]]