View source for 6dzt


==Cryo-EM structure of nucleosome in complex with a single chain antibody fragment==
<SX load='6dzt' size='340' side='right' viewer='molstar' caption='[[6dzt]], [[Resolution|resolution]] 2.99&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6dzt]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster], [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DZT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DZT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.99&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dzt OCA], [https://pdbe.org/6dzt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dzt RCSB], [https://www.ebi.ac.uk/pdbsum/6dzt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dzt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/H3_DROME H3_DROME] 
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== Publication Abstract from PubMed ==
Genomic DNA in eukaryotes is organized into chromatin through association with core histones to form nucleosomes, each distinguished by their DNA sequences and histone variants. Here, we used a single-chain antibody fragment (scFv) derived from the anti-nucleosome antibody mAb PL2-6 to stabilize human CENP-A nucleosome containing a native alpha-satellite DNA and solved its structure by the cryo-electron microscopy (cryo-EM) to 2.6 A resolution. In comparison, the corresponding cryo-EM structure of the free CENP-A nucleosome could only reach 3.4 A resolution. We find that scFv binds to a conserved acidic patch on the histone H2A-H2B dimer without perturbing the nucleosome structure. Our results provide an atomic resolution cryo-EM structure of a nucleosome and insight into the structure and function of the CENP-A nucleosome. The scFv approach is applicable to the structural determination of other native-like nucleosomes with distinct DNA sequences.

Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment.,Zhou BR, Yadav KNS, Borgnia M, Hong J, Cao B, Olins AL, Olins DE, Bai Y, Zhang P Nat Commun. 2019 May 24;10(1):2301. doi: 10.1038/s41467-019-10247-4. PMID:31127102<ref>PMID:31127102</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6dzt" style="background-color:#fffaf0;"></div>

==See Also==
*[[Histone 3D structures|Histone 3D structures]]
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
== References ==
<references/>
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[[Category: Drosophila melanogaster]]
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Yadav KNS]]
[[Category: Zhou B-R]]