View source for 6eml


==Cryo-EM structure of a late pre-40S ribosomal subunit from Saccharomyces cerevisiae==
<SX load='6eml' size='340' side='right' viewer='molstar' caption='[[6eml]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6eml]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EML FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6eml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eml OCA], [https://pdbe.org/6eml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6eml RCSB], [https://www.ebi.ac.uk/pdbsum/6eml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6eml ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS5_YEAST RS5_YEAST] 
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== Publication Abstract from PubMed ==
Mechanistic understanding of eukaryotic ribosome formation requires a detailed structural knowledge of the numerous assembly intermediates, generated along a complex pathway. Here, we present the structure of a late pre-40S particle at 3.6 A resolution, revealing in molecular detail how assembly factors regulate the timely folding of pre-18S rRNA. The structure shows that, rather than sterically blocking 40S translational active sites, the associated assembly factors Tsr1, Enp1, Rio2 and Pno1 collectively preclude their final maturation, thereby preventing untimely tRNA and mRNA binding and error prone translation. Moreover, the structure explains how Pno1 coordinates the 3'end cleavage of the 18S rRNA by Nob1 and how the late factor's removal in the cytoplasm ensures the structural integrity of the maturing 40S subunit.

Cryo-EM structure of a late pre-40S ribosomal subunit from Saccharomyces cerevisiae.,Heuer A, Thomson E, Schmidt C, Berninghausen O, Becker T, Hurt E, Beckmann R Elife. 2017 Nov 20;6. doi: 10.7554/eLife.30189. PMID:29155690<ref>PMID:29155690</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6eml" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
*[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]]
*[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]]
== References ==
<references/>
__TOC__
</SX>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Becker T]]
[[Category: Beckmann R]]
[[Category: Berninghausen O]]
[[Category: Heuer A]]
[[Category: Hurt E]]
[[Category: Schmidt C]]
[[Category: Thomson E]]