View source for 6esp


==Proteome-wide analysis of phospho-regulated PDZ domain interactions==
<StructureSection load='6esp' size='340' side='right'caption='[[6esp]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6esp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ESP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ESP FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SCRIB, CRIB1, KIAA0147, LAP4, SCRB1, VARTUL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6esp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esp OCA], [http://pdbe.org/6esp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6esp RCSB], [http://www.ebi.ac.uk/pdbsum/6esp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6esp ProSAT]</span></td></tr>
</table>
== Disease ==
[[http://www.uniprot.org/uniprot/SCRIB_HUMAN SCRIB_HUMAN]] The disease is caused by mutations affecting the gene represented in this entry. 
== Function ==
[[http://www.uniprot.org/uniprot/SCRIB_HUMAN SCRIB_HUMAN]] Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.<ref>PMID:15182672</ref> <ref>PMID:15775968</ref> <ref>PMID:16344308</ref> <ref>PMID:16965391</ref> <ref>PMID:18641685</ref> <ref>PMID:18716323</ref> <ref>PMID:19041750</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A key function of reversible protein phosphorylation is to regulate protein-protein interactions, many of which involve short linear motifs (3-12 amino acids). Motif-based interactions are difficult to capture because of their often low-to-moderate affinities. Here, we describe phosphomimetic proteomic peptide-phage display, a powerful method for simultaneously finding motif-based interaction and pinpointing phosphorylation switches. We computationally designed an oligonucleotide library encoding human C-terminal peptides containing known or predicted Ser/Thr phosphosites and phosphomimetic variants thereof. We incorporated these oligonucleotides into a phage library and screened the PDZ (PSD-95/Dlg/ZO-1) domains of Scribble and DLG1 for interactions potentially enabled or disabled by ligand phosphorylation. We identified known and novel binders and characterized selected interactions through microscale thermophoresis, isothermal titration calorimetry, and NMR We uncover site-specific phospho-regulation of PDZ domain interactions, provide a structural framework for how PDZ domains accomplish phosphopeptide binding, and discuss ligand phosphorylation as a switching mechanism of PDZ domain interactions. The approach is readily scalable and can be used to explore the potential phospho-regulation of motif-based interactions on a large scale.

Proteome-wide analysis of phospho-regulated PDZ domain interactions.,Sundell GN, Arnold R, Ali M, Naksukpaiboon P, Orts J, Guntert P, Chi CN, Ivarsson Y Mol Syst Biol. 2018 Aug 20;14(8):e8129. PMID:30126976<ref>PMID:30126976</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6esp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human]]
[[Category: Large Structures]]
[[Category: Chi, N C]]
[[Category: Pdz]]
[[Category: Phosphorylation]]
[[Category: Protein binding]]
[[Category: Scribble]]