View source for 6ewn


==HspA from Thermosynechococcus vulcanus in the presence of 2M urea with initial stages of denaturation==
<StructureSection load='6ewn' size='340' side='right'caption='[[6ewn]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ewn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synechococcus_vulcanus Synechococcus vulcanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EWN FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=URE:UREA'>URE</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hspA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32053 Synechococcus vulcanus])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ewn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ewn OCA], [http://pdbe.org/6ewn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ewn RCSB], [http://www.ebi.ac.uk/pdbsum/6ewn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ewn ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Small heat shock proteins (sHSPs) belong to the superfamily of molecular chaperones. They prevent aggregation of partially unfolded or misfolded client proteins, providing protection to organisms under stress conditions. Here, we report the biophysical and structural characterization of a small heat shock protein (HspA) from a thermophilic cyanobacterium Thermosynechococcus vulcanus in the presence of 2M urea. HspA has been shown to be important for the protection of Photosystem II and the Phycobilisome antenna complex at elevated temperatures. Heterologously expressed HspA requires the presence of 1-2M urea to maintain its solubility at concentrations required for most characterization methods. Spectroscopic studies reveal the presence of the beta-sheet structure and intactness of the tertiary fold in HspA. In vitro assays show that the HspA maintains chaperone-like activity in protecting soluble proteins from thermal aggregation. Chromatography and electron microscopy show that the HspA exists as a mixture of oligomeric forms in the presence of 2M urea. HspA was successfully crystallized only in the presence of 2M urea. The crystal structure of HspA shows urea-induced loss of about 30% of the secondary structure without major alteration in the tertiary structure of the protein. The electron density maps reveal changes in the hydrogen bonding network which we attribute to the presence of urea. The crystal structure of HspA demonstrates a mixture of both direct interactions between urea and protein functionalities and interactions between urea and the surrounding solvent that indirectly affect the protein, which are in accordance with previously published studies.

Biophysical and structural characterization of the small heat shock protein HspA from Thermosynechococcus vulcanus in 2 M urea.,Ghosh S, Salama F, Dines M, Lahav A, Adir N Biochim Biophys Acta Proteins Proteom. 2019 Apr;1867(4):442-452. doi:, 10.1016/j.bbapap.2018.12.011. Epub 2019 Jan 31. PMID:30711645<ref>PMID:30711645</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ewn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synechococcus vulcanus]]
[[Category: Adir, N]]
[[Category: Dines, M]]
[[Category: Ghosh, S]]
[[Category: Salama, F]]
[[Category: Chaperone]]
[[Category: Cyanobacteria]]
[[Category: Denaturation]]
[[Category: Small heat shock protein]]
[[Category: Urea]]